| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,490,528 | 0 | G | T | 60.0% | ‑0.4 / 10.8 | 10 | intergenic (+57/+8) | ypdK/alaC | membrane protein YpdK/alanine transaminase |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base T (4/2); total (7/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCC > NZ_CP009273/2490442‑2490610 | tGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGactacg < 2:408532/90‑1 (MQ=255) ggTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACTACGGTGttt > 2:464999/1‑90 (MQ=255) tGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCcgcg > 2:285077/1‑90 (MQ=255) gatgatCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGacgacg > 1:18979/1‑70 (MQ=255) gatgatCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGacgacg < 2:18979/70‑1 (MQ=255) aaCTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACTACGGTGTTTATTCCGCGTTTTCGTGAATATGTTtgctg > 1:262984/1‑90 (MQ=255) aaCTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACTACGGTGTTTATTCCGCGTTTTCGTGAATATGTTtgctg > 1:33101/1‑90 (MQ=255) aaCTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTtgctg > 1:211965/1‑90 (MQ=255) gCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACTACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCg < 2:65581/90‑1 (MQ=255) tCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGGAGTAAACCGTCGGCACg > 1:274666/1‑90 (MQ=255) ccTGTTTGCATTTCTGACTACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATc < 2:33101/90‑1 (MQ=255) tttCTGACTACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATcc > 2:39542/1‑90 (MQ=255) ttCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATccc < 1:54313/90‑1 (MQ=255) | TGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCC > NZ_CP009273/2490442‑2490610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |