| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,118 | 0 | C | A | 66.7% | ‑5.0 / 15.0 | 15 | A422A (GCC→GCA) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (3/2); new base A (0/10); total (3/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCG > NZ_CP009273/3214036‑3214184 | ttgttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGa > 2:422487/1‑90 (MQ=255) gttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATc < 1:515867/90‑1 (MQ=255) gttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATc < 1:52961/90‑1 (MQ=255) tAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCAt > 2:164459/1‑90 (MQ=255) tttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCAAAAACGATCCGCATTGAACCGCcactgacactg < 1:497108/90‑1 (MQ=255) tttGCAAGGGAGTTTTCCCGCCCGCGTGTCCTGGGGGCGGGAACGCCAAAAAACGCAAAAACGATCCGAATTGAACCGCcactgacactg < 1:301799/90‑1 (MQ=255) tttCCCAGGGACTTGTCCCGCCAGCGCGTCGTGGGGGCCGGACCCCTAACTAACGCAAAACCGATCCGCTTTGACCCGCcactgacactg < 2:196211/90‑1 (MQ=255) ggaTGTCCCGCCGGCGCGTATGGGGGGCGGGAACGCTAAAAAACCCAAAAACCCCCCGATTTAACCCGCCACTGACACTGACCATTGAAc < 2:410719/89‑1 (MQ=255) agttGTTCCCCCGGCGCGTCTGGGGGGCCGGAACCCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:210799/87‑1 (MQ=255) taTGTCCCGCCAGCGCTTCGGGGTGGCGGAAACCCTAAAAAACGCAAAACCAACCCCCATTGACCCGCCACTGACACTGACCATTGAACa < 2:278897/89‑1 (MQ=255) ttcccgccgGGGCGTCCGGGGGGCCGGAACGCTAAAAACCCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAgtg < 2:121674/87‑1 (MQ=255) aGCGCGTACTGGGGGCCGGAACCCTCAAAAACCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg < 1:270337/90‑1 (MQ=255) ggtggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgc > 2:213062/1‑90 (MQ=255) ggggCCGAACCGCTAAAAAACCCAAAAACGACCCGCTTTAACCCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcg < 2:183418/88‑1 (MQ=255) gggcccGAAACGCTAAAAACCCCAAAAACGTCCCGAATTAACCCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcg < 2:459706/86‑1 (MQ=255) | TTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCG > NZ_CP009273/3214036‑3214184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |