| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,738,402 | 0 | A | C | 57.1% | 0.5 / 10.0 | 15 | D80A (GAC→GCC) | yiaN | 2,3‑diketo‑L‑gulonate transporter large permease YiaN |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base C (0/8); total (3/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTT > NZ_CP009273/3738315‑3738484 | ccGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGAcc < 1:214254/90‑1 (MQ=255)cagATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGGGGGTAAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGAcc < 1:237802/88‑1 (MQ=255) ttCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATg < 1:206757/90‑1 (MQ=255) tctcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGGGAAAACATGAATGCGGGCGGCTTGTCAAAAACCCTTGTTTACCTGCCGATGa > 2:216601/1‑90 (MQ=255) tgctgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGGGGCTTGTCAAAACGCATTTTTGGCCTGCCGATGAAAAtgg > 1:496209/1‑90 (MQ=255) ctgGCGATTCCTTTTTTTGTGCTGGGGGGTGAAATCATGAAGGGGGGGGGTTTGTCAAAACGCTTTGTTGCCCTGCCGATGAAACtggtg < 2:94175/90‑1 (MQ=255) ctgGCGATTCCGTTTTTTGTGCGGGGGGGTGAAATCATGAATGGGGGGGGCTTGTCAAAACGCATTGTTGCCCTGCCGATGAAACtggtg < 2:117274/90‑1 (MQ=255) tgGCGATTCCGTTTTTTGTGCTGGCGGGTGAAATCATGAATGGGGGCGGCTTGTCAAAACGCATTGTTGCCCTGCCGATGAAACtggtgg < 2:45325/90‑1 (MQ=255) tgGCGATTCCGTTCTTTGTGCTGGCGGGTAAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACtggtgg < 2:173772/90‑1 (MQ=255) ttttGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAACCGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAgg < 1:362254/89‑1 (MQ=255) gccggCGGGTAAAATCATGAACGCGGGCGGCTTGTCAAAACGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCgggct < 1:481811/87‑1 (MQ=255) aaTCATGAATGCGGGCGGCTTGAAAAACCGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCgg < 2:378692/90‑1 (MQ=255) ggcggtTTGTAAAACCGCTTTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCt < 2:318541/90‑1 (MQ=255) cGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGc < 1:57709/90‑1 (MQ=255) aTTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGGGTGCTGGCTACTCTGATTATGGCGAGCCtt > 2:255830/1‑90 (MQ=255) | CCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTT > NZ_CP009273/3738315‑3738484 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |