| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,004,152 | 0 | T | G | 57.1% | 12.3 / 16.2 | 14 | V198G (GTG→GGG) | yigL | sugar/pyridoxal phosphate phosphatase YigL |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG > NZ_CP009273/4004068‑4004241 | aTCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAAGCCATGGGGTGGAAGCGGGGGCga > 1:256493/1‑90 (MQ=255) aGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTAc < 2:362516/90‑1 (MQ=255) aGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTAc < 2:439782/90‑1 (MQ=255) cTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAAGCCATGCGCTGGAAGCGGGGGCGAAGAAACTGGGCTACAGCCTGAAGGa > 1:511168/1‑90 (MQ=255) aaCCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAAGCCATGCGCTGGAAGCGGGGGGGAAGAAACTGGGCTACAGCCTGAAGGATTg > 2:78183/1‑90 (MQ=255) aaCCTGTCTGGAAGTGATGGCGGGCGGCGGTTTAAAAAGCCCTGCGCTGGAAGCGGGGGGGGAGAAAATGGGGTACAGCCTGAAGGAGTg > 2:462174/1‑90 (MQ=255) gTCTGGAAGTGATGGCGGGCGGCGTTTTAAAAGGCCACGCGCTTGAAGCGGGGGCGGAGAAACTGGGGTACAGCCTGAAGGATTTTATTg > 1:275002/1‑90 (MQ=255) gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGGGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTg > 2:362093/1‑90 (MQ=255) gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAAGCCATGCGCTGGGAGCGGGGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTg > 2:415069/1‑90 (MQ=255) gAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGttt < 1:362093/90‑1 (MQ=255) gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGa < 1:462174/90‑1 (MQ=255) gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGGGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGa > 1:403967/1‑90 (MQ=255) ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAac < 1:45618/90‑1 (MQ=255) aTGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGt > 2:378501/1‑90 (MQ=255) tGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTAATGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAg > 2:503288/1‑90 (MQ=255) | ATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG > NZ_CP009273/4004068‑4004241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |