| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,951 | 0 | G | C | 61.5% | 3.4 / 11.2 | 14 | A59G (GCG→GGG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (2/3); new base C (0/8); total (3/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058864‑4059038 | ggACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGcc < 1:17451/90‑1 (MQ=255) gACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGcca < 1:79714/90‑1 (MQ=255) gCCTTGAGGCCAAATATTTTCCCGCGAGTGGAGAAAAAACCCGGTGAGCATATCGGTAAACGGGGAAAAAACCTCCCCCACCAAAAAGaa < 2:341313/90‑2 (MQ=255) tttAGCCCAAATTTTTTCCCGCGAGTCGAGAAAAAATCCGGTGAGCATATCGGTAAACGCGGAAAAAACCTTCCCCACCAAAAAGATaac < 2:101943/90‑2 (MQ=255) gccccAATTTTTTCCCGCGAGTCGAGAAAAAACCGGGTGAGCATATCGGAAAACGCGGAAAAAACCTTCCCCACCAAAAAGATAATCCCg < 2:5683/88‑1 (MQ=255) gCCAAATATTTTCCCGCGAGTCGAGAAAAAACCCGGTGAGCATATCGGAAACCGCGGAAAAAACCTTCGCCACAAAAAAGATAATCCCGc < 2:303264/90‑1 (MQ=255) cgcgAGTCGAGAAAAAACCGGGTGAGCATATGGGTAAACGGGGAAAAAAACTTCCCCCCCAGAAAGATTACCCCGCCATAGTAAGCAGGc < 1:476020/90‑1 (MQ=255) gcgAGTGGAAAAAAAACCGGGTGAGCATATCGGAAAACGGGGAAAAAAACTCCCCCCCCAAAAAAATAACCCCGCCATAGTAAGCAGGCa < 2:76617/90‑1 (MQ=255) aaaaCCCGGTGACAATATCGAAAAACGCGAAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCAt < 2:61045/90‑1 (MQ=255) aaaCCCGGGGACCATATCGGAAAACGCGGAAAAAACCTCCCCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 2:364860/90‑1 (MQ=255) aacccGGTGAGCATACGGGAAAACGCGGAAAAAACCTCCCCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg < 2:83694/87‑1 (MQ=255) cGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtat > 1:61045/1‑90 (MQ=255) ggTGAGCATATAGGAAAACGCGGTAACAACCATCACCATGAGATGGATATTCCAGACATAGGACGCAGGCATCGCTAATTCATCGGtata > 2:426582/1‑90 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGGATAAAATTTGAGGAGATACAGAGGACCGATGCaga > 1:304346/1‑90 (MQ=255) | GGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058864‑4059038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |