Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,725	T→C	D463D (GAT→GAC)	thrA →	bifunctional aspartate kinase/homoserine dehydrogenase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,725	0	T	C	100.0%	23.5 / NA	8	D463D (GAT→GAC)	thrA	bifunctional aspartate kinase/homoserine dehydrogenase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/2);  total (6/2)

CTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCA  >  NZ_CP009273/1647‑1796                                                                               |                                                                        cTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGACCAGGTTATCGa                                                              >  2:255314/1‑90 (MQ=255) cTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGACCAGGTTATCGa                                                              >  2:325133/1‑90 (MQ=255) cTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGACCAGGTTATCGa                                                              <  2:327093/90‑1 (MQ=255)                           atgatgCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGACCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCg                                    >  2:412079/1‑90 (MQ=255)                                  gACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGACCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCgg                             >  1:243035/1‑90 (MQ=255)                                                             gATGCTGTTCAATACCGACCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGc               <  1:443708/77‑1 (MQ=255)                                                             gATGCTGTTCAATACCGACCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGc               >  2:443708/1‑77 (MQ=255)                                                             gATGCTGTTCAATACCGACCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCa  >  1:263953/1‑90 (MQ=255)                                                                               |                                                                        CTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCA  >  NZ_CP009273/1647‑1796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑