Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	230,562	T→C	Q236Q (CAA→CAG)	gloB ←	hydroxyacylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	230,562	0	T	C	100.0%	16.2 / NA	6	Q236Q (CAA→CAG)	gloB	hydroxyacylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/0);  total (6/0)

CATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCG  >  NZ_CP009273/230477‑230639                                                                                      |                                                                              cATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGgctgtt                                                                           <  1:475459/90‑1 (MQ=255)         cGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGCTGTTGCaataat                                                                   >  2:426011/1‑90 (MQ=255)          gAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGCTGTTGCAATAATg                                                                  >  2:362298/1‑90 (MQ=255)                aGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGCTGTTGCAATAATGTttctt                                                            >  2:307535/1‑90 (MQ=255)                                                                   aTGCAAAACGCTCTTCAGGCTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTaa         >  2:454111/1‑90 (MQ=255)                                                                   aTGCAAAACGCTCTTCAGGCTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTaa         >  2:480384/1‑90 (MQ=255)                                                                          aCGCTCTTCAGGCTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCg  >  2:150909/1‑90 (MQ=255)                                                                                      |                                                                              CATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAACATTAATTTGCCG  >  NZ_CP009273/230477‑230639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑