Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,912,667	A→T	M301L (ATG→TTG)	yebT →	lipid‑binding membrane homeostasis protein YebT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,912,667	0	A	T	100.0%	54.1 / NA	15	M301L (ATG→TTG)	yebT	lipid‑binding membrane homeostasis protein YebT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/6);  total (9/6)

CTGGCCCACAGCCAGCGTGGCGTAATAATAAAACTGGAACTGCCGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTAATGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGATCC  >  NZ_CP009273/1912586‑1912752                                                                                  |                                                                                      cTGGCCCACAGCCAGCGTGGCGTAATAATAAAACTGGAACTGCCGCGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAg                                                                               >  1:260469/1‑90 (MQ=255) cTGGCCCACAGCCAGCGTGGCGTAATAATAAAACTGGAACTGCCGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAg                                                                               >  1:202770/1‑90 (MQ=255) cTGGCCCACAGCCAGCGTGGCGTAATAATAAAACTGGAACTGCCGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAg                                                                               >  1:53494/1‑90 (MQ=255) cTGGCCCACAGCCAGCGTGGCGTAATAATAAAACTGGAACTGCCGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAg                                                                               >  2:110099/1‑90 (MQ=255) cTGGCCCACAGCCAGCGTGGCGTAATAATAAAACTGGAACTGCCGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAg                                                                               >  2:305555/1‑90 (MQ=255) cTGGCCCACAGCCAGCGTGGCGTAATAATAAAACTGGAACTGCCGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAg                                                                               >  2:388885/1‑90 (MQ=255)          agccagcGTGGCGTAATAATAAAACTGGAACTGCCGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAGGGGCTGGaa                                                                      <  1:10201/90‑1 (MQ=255)               gcGTGGCGTAATAATAAAACTGGAACTGCCGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAGGGGCTGGAAGTCgg                                                                 <  2:421891/90‑1 (MQ=255)                                    ggAACTGCCGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGAttt                                            >  2:150231/1‑90 (MQ=255)                                           ccGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCt                                     <  1:228389/90‑1 (MQ=255)                                                   ggCCGGATTAACCGCCGACTCGACGCCGTTATTGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTaa                             <  1:130993/90‑1 (MQ=255)                                                            aaCCGCCGACTCGACGCCGTTATTGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAgg                    >  1:7239/1‑90 (MQ=255)                                                                 ccgACTCGACGCCGTTATTGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGaaa               >  1:182505/1‑90 (MQ=255)                                                                          cGCCGTTATTGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTg      <  1:78348/90‑1 (MQ=255)                                                                              gTTATTGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGAccc  <  2:258650/90‑1 (MQ=255)                                                                                  |                                                                                      CTGGCCCACAGCCAGCGTGGCGTAATAATAAAACTGGAACTGCCGAGTGGGGCCGGATTAACCGCCGACTCGACGCCGTTAATGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGATCC  >  NZ_CP009273/1912586‑1912752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑