| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,912,750 | T→C | D328D (GAT→GAC) | yebT → | lipid‑binding membrane homeostasis protein YebT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,912,750 | 0 | T | C | 100.0% | 62.3 / NA | 18 | D328D (GAT→GAC) | yebT | lipid‑binding membrane homeostasis protein YebT |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/8); total (10/8) | |||||||||||
GTTAATGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGATCCCAGCGTCGTTACCCTGCTTCGTGAAAATACCCGCATCGAATTACGCAACCCGAAATTATCCCTTAGCGATGCTAATCT > NZ_CP009273/1912663‑1912830 | gTTATTGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGAccc < 2:258650/90‑1 (MQ=255) ttGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGc < 2:260469/89‑1 (MQ=255) tATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGcgtc < 1:289161/90‑1 (MQ=255) aTCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGc < 2:416122/86‑1 (MQ=255) aTCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGc > 1:416122/1‑86 (MQ=255) cAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGc < 2:96171/84‑1 (MQ=255) cAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGc > 1:96171/1‑84 (MQ=255) cTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGc > 1:43804/1‑78 (MQ=255) cTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGc < 2:43804/78‑1 (MQ=255) cTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGCGTCGTTACCCTg > 2:161291/1‑90 (MQ=255) gTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGCGTCGTTACCCTGCTTCGt > 2:306531/1‑90 (MQ=255) tAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGCGTCGTTACCCTGCTTCGTGAAAATACCCGCATCGAATTACGCa > 1:396730/1‑90 (MQ=255) cTGGTGGTAAAGTCACAGGGGAAATGACCGTTGACCCCAGCGTCGTTACCCTGCTTCGTGAAAATACCCGCATCGAATTACGCAACCCGa > 2:84694/1‑90 (MQ=255) tggtggTAAAGTCACAGGGGAAATGACCGTTGACCCCAGCGTCGTTACCCTGCTTCGTGAAAATACCCGCATCGAATTACGCAACCCGaa > 1:357844/1‑90 (MQ=255) acaGGGGAAATGACCGTTGACCCCAGCGTCGTTACCCTGCTTCGTGAAAATACCCGCATCGAATTACGCAACCCGAAATTATCCCTTAGc < 2:182505/90‑1 (MQ=255) acaGGGGAAATGACCGTTGACCCCAGCGTCGTTACCCTGCTTCGTGAAAATACCCGCATCGAATTACGCAACCCGAAATTATCCCTTAGc < 1:306531/90‑1 (MQ=255) acaGGGGAAATGACCGTTGACCCCAGCGTCGTTACCCTGCTTCGTGAAAATACCCGCATCGAATTACGCAACCCGAAATTATCCCTTAGc < 1:150231/90‑1 (MQ=255) aaaTGACCGTTGACCCCAGCGTCGTTACCCTGCTTCGTGAAAATACCCGCATCGAATTACGCAACCCGAAATTATCCCTTAGCGATGCTa > 1:417551/1‑90 (MQ=255) gACCGTTGACCCCAGCGTCGTTACCCTGCTTCGTGAAAATACCCGCATCGAATTACGCAACCCGAAATTATCCCTTAGCGATGCTAAtct > 2:481339/1‑90 (MQ=255) | GTTAATGTATCAGGGGCTGGAAGTCGGACAGCTGACTAAACTGGATTTAAATCCTGGTGGTAAAGTCACAGGGGAAATGACCGTTGATCCCAGCGTCGTTACCCTGCTTCGTGAAAATACCCGCATCGAATTACGCAACCCGAAATTATCCCTTAGCGATGCTAATCT > NZ_CP009273/1912663‑1912830 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |