| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,914,076 | T→G | G770G (GGT→GGG) | yebT → | lipid‑binding membrane homeostasis protein YebT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,914,076 | 0 | T | G | 95.2% | 68.6 / ‑3.7 | 21 | G770G (GGT→GGG) | yebT | lipid‑binding membrane homeostasis protein YebT |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (10/10); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCGTTACCTGGATGGCTTAAGCATTATTGTTGAAGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGTCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGACATTGTCAGATCGCGTGATGATTGCGATGCGCATCAGTAAACGCTATC > NZ_CP009273/1913992‑1914161 | gcgTTACCTGGATGGCTTAAGCATTATTGTTGAAGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGa > 2:234014/1‑90 (MQ=255)gcgTTACCTGGATGGCTTAAGCATTATTGTTGAAGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGa > 1:27537/1‑90 (MQ=255) gTTACCTGGATGGCTTAAGCATTATTGTTGAAGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAg < 1:358585/90‑1 (MQ=255) tACCTGGATGGCTTAAGCATTATTGTTGAAGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTc < 1:257027/90‑1 (MQ=255) tGGATGGCTTAAGCATTATTGTTGAAGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTa < 1:283133/90‑1 (MQ=255) aGCATTATTGTTGAAGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGa > 1:454573/1‑90 (MQ=255) ttattGTTGAAGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGTCTGGAAGTCGGTACGGTTACAGGAATGa > 1:263713/1‑90 (MQ=255) ttattGTTGAAGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGa > 1:211278/1‑90 (MQ=255) aGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACa < 1:80218/73‑1 (MQ=255) aGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACa > 2:80218/1‑73 (MQ=255) aGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGAc < 2:310658/90‑1 (MQ=255) aGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGAc < 2:485815/90‑1 (MQ=255) aGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGAc > 2:487500/1‑90 (MQ=255) gCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGACATTGTCAGAt < 1:487500/90‑1 (MQ=255) tCGGTACGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGACATTGTCAGATCGCGTGATGATTGCGa > 2:86857/1‑90 (MQ=255) aCGCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGACATTGTCAGATCGCGTGATGATTGCGATgcgc < 1:304192/90‑1 (MQ=255) gCCTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGACATTGTCAGATCGCGTGATGATTGCGATGCGCAt < 1:234014/90‑1 (MQ=255) ccTGTGCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGACATTGTCAGATCGCGTGATGATTGCGATGCGCATc < 1:203093/90‑1 (MQ=255) gtgCTGTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGACATTGTCAGATCGCGTGATGATTGCGATGCGCATCAGt > 2:75885/1‑90 (MQ=255) gTTCCGTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGACATTGTCAGATCGCGTGATGATTGCGATGCGCATCAGTAAACg > 1:476738/1‑90 (MQ=255) gTGGGCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGACATTGTCAGATCGCGTGATGATTGCGATGCGCATCAGTAAACGCTATc > 1:404523/1‑90 (MQ=255) | GCGTTACCTGGATGGCTTAAGCATTATTGTTGAAGCGCCGGAAGCCGGTTCGTTAGGCATCGGTACGCCTGTGCTGTTCCGTGGTCTGGAAGTCGGTACGGTTACAGGAATGACGCTGGGGACATTGTCAGATCGCGTGATGATTGCGATGCGCATCAGTAAACGCTATC > NZ_CP009273/1913992‑1914161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |