Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,937,480	A→C	T188P (ACC→CCC)	znuC →	zinc ABC transporter ATP‑binding protein ZnuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,937,480	0	A	C	100.0%	73.2 / NA	21	T188P (ACC→CCC)	znuC	zinc ABC transporter ATP‑binding protein ZnuC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/8);  total (13/8)

TTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATT  >  NZ_CP009273/1937393‑1937560                                                                                        |                                                                                 ttATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAccc                                                                                <  2:104584/90‑1 (MQ=255)        aCCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAg                                                                         >  2:479791/1‑90 (MQ=255)            tATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCt                                                                     >  1:283296/1‑90 (MQ=255)            tATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCt                                                                     >  2:210415/1‑90 (MQ=255)                gACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGc                                                                 <  2:300504/90‑1 (MQ=255)                  ccAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCt                                                               >  1:518331/1‑90 (MQ=255)                   cAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTg                                                              >  2:396355/1‑90 (MQ=255)                        gCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATca                                                         <  2:103660/90‑1 (MQ=255)                          gtcgtGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATcacc                                                       <  1:418262/90‑1 (MQ=255)                                         gtgGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCg                                        >  1:166014/1‑90 (MQ=255)                                           ggCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGc                                      >  2:371318/1‑90 (MQ=255)                                              gTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGcaca                                   >  2:319318/1‑90 (MQ=255)                                                        tttCTCACGACCTCCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAgctg                         >  2:317953/1‑90 (MQ=255)                                                                gATCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTccccg                 >  1:462645/1‑88 (MQ=255)                                                                  tCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCa               <  1:210415/90‑1 (MQ=255)                                                                     gCATCTGGTGATGACAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATcc            >  2:244996/1‑90 (MQ=255)                                                                       aTCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCgg          <  2:202634/90‑1 (MQ=255)                                                                       aTCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCgg          <  1:396355/90‑1 (MQ=255)                                                                          tGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGt       <  1:371318/90‑1 (MQ=255)                                                                           ggTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGtt      >  2:99465/1‑90 (MQ=255)                                                                           ggTGATGACAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGtt      <  1:244996/90‑1 (MQ=255)                                                                               aTGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGATGTTGTTTCCCCGCATCCGGAGTTTAtt  >  1:69938/1‑90 (MQ=255)                                                                                        |                                                                                 TTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATT  >  NZ_CP009273/1937393‑1937560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑