Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,937,544	T→C	L209P (CTG→CCG)	znuC →	zinc ABC transporter ATP‑binding protein ZnuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,937,544	0	T	C	100.0%	105.2 / NA	29	L209P (CTG→CCG)	znuC	zinc ABC transporter ATP‑binding protein ZnuC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/16);  total (13/16)

TCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACG  >  NZ_CP009273/1937458‑1937627                                                                                       |                                                                                    tCTGCATCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCa                                                                                  <  1:210415/90‑1 (MQ=255)    gCATCTGGTGATGACAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATcc                                                                               >  2:244996/1‑90 (MQ=255)      aTCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCgg                                                                             <  2:202634/90‑1 (MQ=255)      aTCTGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCgg                                                                             <  1:396355/90‑1 (MQ=255)         tGGTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGt                                                                          <  1:371318/90‑1 (MQ=255)          ggTGATGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGtt                                                                         >  2:99465/1‑90 (MQ=255)          ggTGATGACAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGtt                                                                         <  1:244996/90‑1 (MQ=255)              aTGGCAAAACCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGATGTTGTTTCCCCGCATCCGGAGTTTAtt                                                                     >  1:69938/1‑90 (MQ=255)                                gTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCgt                                                   <  1:147624/90‑1 (MQ=255)                                gTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCgt                                                   <  2:112846/90‑1 (MQ=255)                                     gtgCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGc                                              >  2:108611/1‑90 (MQ=255)                                       gCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTg                                            >  1:328311/1‑90 (MQ=255)                                             aTCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGaacaac                                      <  2:283296/90‑1 (MQ=255)                                             aTCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGaacaac                                      <  2:24934/90‑1 (MQ=255)                                               caccacATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTg                                    <  1:27188/90‑1 (MQ=255)                                                 ccacATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTggg                                  >  2:97160/1‑90 (MQ=255)                                                     aTTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGttt                                                                        <  1:142458/48‑1 (MQ=255)                                                     aTTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGttt                                                                        >  2:142458/1‑48 (MQ=255)                                                      tttGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGtatct                             >  1:268051/1‑90 (MQ=255)                                                           tgttCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGc                        >  2:4888/1‑90 (MQ=255)                                                             ttCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCca                      <  1:99465/90‑1 (MQ=255)                                                              tCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCcat                     >  2:166387/1‑90 (MQ=255)                                                              tCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCcat                     >  2:460593/1‑90 (MQ=255)                                                              tCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCcat                     <  1:166387/90‑1 (MQ=255)                                                              tCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCcat                     >  2:12627/1‑90 (MQ=255)                                                               ccGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCcatc                    <  2:13938/90‑1 (MQ=255)                                                               ccGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCcatc                    <  2:496663/90‑1 (MQ=255)                                                                               tgttTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCa    >  1:504852/1‑90 (MQ=255)                                                                                 tttCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACg  <  1:317953/90‑1 (MQ=255)                                                                                       |                                                                                    TCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACG  >  NZ_CP009273/1937458‑1937627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑