Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,963,824	A→G	V473A (GTC→GCC)	tap ←	methyl‑accepting chemotaxis protein IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,963,824	0	A	G	100.0%	51.0 / NA	15	V473A (GTC→GCC)	tap	methyl‑accepting chemotaxis protein IV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/11);  total (4/11)

TCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCAC  >  NZ_CP009273/1963737‑1963909                                                                                        |                                                                                      tCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGGCa                                                                                     <  1:380187/90‑1 (MQ=255) tCGCCCTGATCCGCCAGTTGTTCCGTTCCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGGCa                                                                                     <  2:312258/90‑1 (MQ=255)   ggCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGGCAGc                                                                                   <  1:249459/90‑1 (MQ=255)   ggCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGGCAGc                                                                                   <  1:397911/90‑1 (MQ=255)   gcccTGTTTCCCCAGTTTTTCCGTTCCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGGCAGc                                                                                   <  2:365238/88‑1 (MQ=255)    gCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGGCAGcc                                                                                  <  1:54338/90‑1 (MQ=255)              ccAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGGCAGCCTGTGCAACCt                                                                        >  1:317269/1‑90 (MQ=255)                          ttGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGGCAGCCTGTGCAACCTGCTCAATccccc                                                            <  2:87195/90‑1 (MQ=255)                                   ccgcTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGGCAGCCTGTGCAACCTGCTCAATCCCCCGCTgttgtt                                                   <  1:320975/90‑1 (MQ=255)                                                         gTTCTGCTGAGTCACCTGATCCATCTGGCTGGCAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTtc                             <  2:466646/90‑1 (MQ=255)                                                          ttCTGCTGAGTCACCTGATCCATCTGGCTGGCAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTtct                            <  1:60511/90‑1 (MQ=255)                                                                 gAGTCACCTGATCCATCTGGCTGGCAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATaa                     >  2:191782/1‑90 (MQ=255)                                                                   gTCACCTGATCCATCTGGCTGGCAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATg                   >  2:458755/1‑90 (MQ=255)                                                                            tCCATCTGGCTGGCAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCAcg          <  2:317269/90‑1 (MQ=255)                                                                                    gCTGGCAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCAc  >  2:270308/1‑90 (MQ=255)                                                                                        |                                                                                      TCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCAC  >  NZ_CP009273/1963737‑1963909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑