Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	232,843	T→G	I97S (ATC→AGC)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	232,843	0	T	G	100.0%	34.7 / NA	11	I97S (ATC→AGC)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/5);  total (6/5)

GCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAA  >  NZ_CP009273/232758‑232918                                                                                      |                                                                            gCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGAGCCAt                                                                         >  1:489796/1‑90 (MQ=255)        aaTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGAGCCATAACGCAg                                                                  <  1:228259/90‑1 (MQ=255)                                       aGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGAGCCATAACGCAGCGTTCGATATc                                                       <  1:351592/70‑1 (MQ=255)                                       aGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGAGCCATAACGCAGCGTTCGATATc                                                       >  2:351592/1‑70 (MQ=255)                                                             tataTTCGCGGCGCGGAGTTGGTGAGCCATAACGCAGCGTTc                                                             >  1:203595/1‑42 (MQ=255)                                                             tataTTCGCGGCGCGGAGTTGGTGAGCCATAACGCAGCGTTc                                                             <  2:203595/42‑1 (MQ=255)                                                             tataTTCGCGGCGCGGAGTTGGTGAGCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt             >  1:239797/1‑90 (MQ=255)                                                             tataTTCGCGGCGCGGAGTTGGTGAGCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt             >  1:448863/1‑90 (MQ=255)                                                                 ttCGCGGCGCGGAGTTGGTGAGCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa         <  2:203179/90‑1 (MQ=255)                                                                   cgcggcgcggAGTTGGTGAGCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAg       <  2:148663/90‑1 (MQ=255)                                                                        cgcggAGTTGGTGAGCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCaa  >  2:123943/1‑90 (MQ=255)                                                                                      |                                                                            GCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAA  >  NZ_CP009273/232758‑232918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑