Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,148,643	A→G	T383A (ACC→GCC)	mdtA →	multidrug efflux RND transporter subunit MdtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,148,643	0	A	G	100.0%	61.2 / NA	18	T383A (ACC→GCC)	mdtA	multidrug efflux RND transporter subunit MdtA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/5);  total (13/5)

GGCATTCAGGACAGTCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGA  >  NZ_CP009273/2148559‑2148698                                                                                     |                                                        ggCATTCAGGACAGTCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGaa                                                    >  1:348581/1‑90 (MQ=255)       cAGGACAGTCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCg                                              <  1:311602/90‑1 (MQ=255)              gTCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAaagtgg                                       >  2:384462/1‑90 (MQ=255)              gTCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAaagtgg                                       >  2:226730/1‑90 (MQ=255)              gTCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAaagtgg                                       >  1:57573/1‑90 (MQ=255)                        ggtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAaagtggaagtg                                  >  2:479615/1‑85 (MQ=255)                        ggtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAaagtggaagtg                                  <  1:479615/85‑1 (MQ=255)                        ggtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGa                             >  1:297102/1‑90 (MQ=255)                        ggtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGa                             >  1:65427/1‑90 (MQ=255)                        ggtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGa                             >  1:394954/1‑90 (MQ=255)                        ggtgATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGa                             >  1:297415/1‑90 (MQ=255)                                          ttCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCactac           >  2:120745/1‑90 (MQ=255)                                               cGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCgg      >  2:45900/1‑90 (MQ=255)                                                  gCGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAag   >  1:486752/1‑90 (MQ=255)                                                   cGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAaga  <  2:469017/90‑1 (MQ=255)                                                   cGATCGCGTGGTGACAGACGGCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAaga  <  1:176985/90‑1 (MQ=255)                                                                      ggCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCact             <  1:500761/60‑1 (MQ=255)                                                                      ggCATTGATCGCCTGGCCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCact             >  2:500761/1‑60 (MQ=255)                                                                                     |                                                        GGCATTCAGGACAGTCAGAAAGTGGTGATCCGTGCAGGTATTTCTGCGGGCGATCGCGTGGTGACAGACGGCATTGATCGCCTGACCGAAGGGGCGAAAGTGGAAGTGGTGGAAGCCCAGAGCGCCACTACTCCGGAAGA  >  NZ_CP009273/2148559‑2148698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑