Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,173,405	A→G	E369G (GAA→GGA)	yegT →	nucleoside permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,173,405	0	A	G	100.0%	30.5 / NA	10	E369G (GAA→GGA)	yegT	nucleoside permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/5);  total (5/5)

TGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGC  >  NZ_CP009273/2173318‑2173482                                                                                        |                                                                              tGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGAAAAGATGTTCGCTTATCAGGGAc                                                                             >  2:73778/1‑90 (MQ=255)        gCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGAAAAGATGTTCGCTTATCAGGGACCGGTaaa                                                                      >  1:60392/1‑90 (MQ=255)                   aGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGAAAAGATGTTCGCTTATCAGGGACCGGTAAACGGACTGACtt                                                           <  2:60392/90‑1 (MQ=255)                     ggCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGAAAAGATGTTCGCTTATCAGGGACCGGTAAACGGACTGACTTTc                                                         >  1:317837/1‑90 (MQ=255)                        ttCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGAAAAGATGTTCGCTTATCAGGGACCGGTAAACGGACTGACTTTCAAc                                                      <  2:317837/90‑1 (MQ=255)                        ttCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGAAAAGATGTTCGCTTATCAGGGACCGGTAAACGGACTGACTTTCAAc                                                      <  2:389519/90‑1 (MQ=255)                         tCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGAAAAGATGTTCGCTTATCAGGGACCGGTAAACGGACTGACTTTCAACt                                                     <  1:121469/90‑1 (MQ=255)                                                                   aaaaGATGTTCGCTTATCAGGGACCGGTAAACGGACTGACTTTCAACTGGTCGGGGATGAGGACTTTCGGCGCGGTGATGATTGCCATTa           >  1:369567/1‑90 (MQ=255)                                                                          gTTCGCTTATCAGGGACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGt    <  1:167148/90‑1 (MQ=255)                                                                            tCGCTTATCAGGGACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGc  >  1:389790/1‑90 (MQ=255)                                                                                        |                                                                              TGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGC  >  NZ_CP009273/2173318‑2173482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑