Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,192,420	A→G	pseudogene (2468/3796 nt)	BW25113_RS25680 →	WGR and DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,192,420	0	A	G	100.0%	35.2 / NA	11	pseudogene (2468/3796 nt)	BW25113_RS25680	WGR and DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/6);  total (5/6)

ATTAAAAGCAAATGCCCAATCACTGCCAGATAGCGCCCTCCTCCACCTCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTATCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCT  >  NZ_CP009273/2192334‑2192486                                                                                       |                                                                   aTTAAAAGCAAATGCCCAATCACTGCCAGATAGCGCCCTCCTCCCCCTCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTGTcc                                                                 <  1:449650/90‑1 (MQ=255)         cAAATGCCCAATCACTGCCAGATAGCGCCCTCCTCCACCTCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTGTCCg                                                                >  1:468846/1‑83 (MQ=255)         cAAATGCCCAATCACTGCCAGATAGCGCCCTCCTCCACCTCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTGTCCg                                                                <  2:468846/83‑1 (MQ=255)         cAAATGCCCAATCACTGCCAGATAGCGCCCTCCTCCACCTCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTGTCCGGGattat                                                         >  2:5012/1‑90 (MQ=255)              gCCCAATCACTGCCAGATAGCGCCCTCCTCCACCTCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTGTCCGGGATTATTGCAg                                                    <  1:309930/90‑1 (MQ=255)                                 gcgcCCTCCTCCACCTCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTGTCCGGGATTATTGCAGGTGAAAGACGTCTGTTccg                                 >  2:315299/1‑90 (MQ=255)                                 gcgcCCTCCTCCACCTCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTGTCCGGGATTATTGCAGGTGAAAGACGTCTGTTccg                                 >  2:68552/1‑90 (MQ=255)                                          tccACCTCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTGTCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCAc                        <  2:343329/90‑1 (MQ=255)                                                tCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTGTCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCgg                  >  1:167492/1‑90 (MQ=255)                                                                cTTCCCTCAGGAAGAGGCTCTGTGTCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCt  <  1:304185/90‑1 (MQ=255)                                                                cTTCCCTCAGGAAGAGGCTCTGTGTCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCt  <  2:328707/90‑1 (MQ=255)                                                                                       |                                                                   ATTAAAAGCAAATGCCCAATCACTGCCAGATAGCGCCCTCCTCCACCTCGGTGAAATGCTCCGCTTCCCTCAGGAAGAGGCTCTGTATCCGGGATTATTGCAGGTGAAAGACGTCTGTTCCGCCGACTCACTGGCGGGATTTGCCTGGGATCT  >  NZ_CP009273/2192334‑2192486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑