Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,236,596	A→C	V179G (GTG→GGG)	folE ←	GTP cyclohydrolase I FolE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,236,596	0	A	C	100.0%	39.1 / NA	12	V179G (GTG→GGG)	folE	GTP cyclohydrolase I FolE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (4/8);  total (4/8)

TGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGC  >  NZ_CP009273/2236513‑2236679                                                                                    |                                                                                    tGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCCCCGCGt                                                                               <  1:158097/90‑1 (MQ=255) tGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCCCCGCGt                                                                               <  1:162304/90‑1 (MQ=255)        gAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCCCCGCGtcgatcga                                                                        >  2:395076/1‑90 (MQ=255)               ccaccaAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCCCCGCGTCGATCGAGACAGcc                                                                 <  2:16792/90‑1 (MQ=255)                  ccaAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCCCCGCGTCGATCGAGACAGCCACg                                                              >  1:230573/1‑90 (MQ=255)                               ttGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCCCCGCGtcgatc                                                                          <  1:383897/65‑1 (MQ=255)                               ttGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCCCCGCGtcgatc                                                                          >  2:383897/1‑65 (MQ=255)                                    gTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCCCCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCagcagc                                            >  2:519517/1‑90 (MQ=255)                                             gTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCCCCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAgc                                   <  2:99621/90‑1 (MQ=255)                                                    cgcgGATGCCACGCGCCTTCACGCAGTAATGCCCCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATaa                            <  2:230573/90‑1 (MQ=255)                                                                              tAATGCCCCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGc  <  2:255622/90‑1 (MQ=255)                                                                              tAATGCCCCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGc  <  2:35875/90‑1 (MQ=255)                                                                                    |                                                                                    TGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGC  >  NZ_CP009273/2236513‑2236679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑