Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,241,916	A→G	G32G (GGT→GGC)	lysP ←	lysine‑specific permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,241,916	0	A	G	100.0%	17.3 / NA	8	G32G (GGT→GGC)	lysP	lysine‑specific permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/3);  total (5/3)

AATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTG  >  NZ_CP009273/2241838‑2241989                                                                               |                                                                          aaTCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTGCCGATGGAAcc                                                                >  1:373736/1‑90 (MQ=255)          ataGGAGAACAACCCCCCCCCCGGCCCTGCCAAAAAAATCGTGGCGCAAGAGGCAAAAAAAAGCCCTGTGCCGATGAAACCGCCAATGGc                                                       <  2:241004/90‑1 (MQ=255)                gagCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTGCCGATGGAACCGCCAATGGCAatcat                                                 >  2:383540/1‑90 (MQ=255)                gagCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTGCCGATGGAACCGCCAATGGCAatcac                                                 >  1:8860/1‑89 (MQ=255)                gaccAAGGCCCCGCCCGGACCTGCCTAAGAAATCGTTGCGCCAGAGGCAAAAAAAAGCCCTGTGCGGATGGAACCGCCAATGGCAatcat                                                 <  1:466883/87‑1 (MQ=255)                  gCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTGCCGATGGAACCGCCAATGGCAATCATCg                                               >  1:8771/1‑90 (MQ=255)                                 gACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTGCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCt                                >  2:345635/1‑90 (MQ=255)                                                               caaaaaAAAGCCCTGTGCCGAGGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCtg  <  1:468334/90‑1 (MQ=255)                                                                               |                                                                          AATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTG  >  NZ_CP009273/2241838‑2241989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑