Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,413,360	A→G	V202A (GTC→GCC)	yfcF ←	glutathione transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,413,360	0	A	G	100.0%	85.7 / NA	25	V202A (GTC→GCC)	yfcF	glutathione transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/15);  total (10/15)

CCTCGTCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGCACCTCATCGCCATGTAGTACCAGGC  >  NZ_CP009273/2413273‑2413435                                                                                        |                                                                            cctcGTCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCa                                                                           >  1:447588/1‑90 (MQ=255)   tcgtcgAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGa                                                                         >  1:163453/1‑90 (MQ=255)    cgtcgAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGAc                                                                        <  1:411628/90‑1 (MQ=255)                   aGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTgg                                                         <  1:356827/90‑1 (MQ=255)                             cTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTg                                                                >  2:55630/1‑73 (MQ=255)                             cTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTg                                                                <  1:55630/73‑1 (MQ=255)                             cTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCAt                                               >  1:175235/1‑90 (MQ=255)                              tGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATa                                              >  2:51906/1‑90 (MQ=255)                               ggATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGt                                                    >  1:476581/1‑83 (MQ=255)                               ggATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGt                                                    <  2:476581/83‑1 (MQ=255)                                 aTTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATc                                           >  1:2208/1‑90 (MQ=255)                                 aTTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATc                                           >  2:384643/1‑90 (MQ=255)                                         gTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGc                                   >  2:264035/1‑90 (MQ=255)                                         gTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGc                                   <  1:384643/90‑1 (MQ=255)                                         gTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGc                                   <  1:337635/90‑1 (MQ=255)                                           tGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGt                                 >  2:416841/1‑90 (MQ=255)                                                 cAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGc                           <  1:264042/90‑1 (MQ=255)                                                           gCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGCACCTCATCGc                 <  2:163453/90‑1 (MQ=255)                                                           gCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGCACCTCATCGc                 <  2:12871/90‑1 (MQ=255)                                                           gCTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGCACCTCATCGc                 <  2:416029/90‑1 (MQ=255)                                                            cTTCGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGCACCTCATCGcc                <  2:2208/90‑1 (MQ=255)                                                               cGCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGCACCTCATCGCCATg             <  2:175235/90‑1 (MQ=255)                                                                gCCGAAAGTGCAATAAAACGTTGGGCAGCCCCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGCCCCTCATCGCCATgt            <  2:337136/90‑1 (MQ=255)                                                                gCCGAAAGTGCAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGCACCTCATCGCCATgt            <  1:416841/90‑1 (MQ=255)                                                                          cAATAAAACGCTGGGCAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGCACCTCATCGCCATGTAGTACCAGGc  <  2:499427/90‑1 (MQ=255)                                                                                        |                                                                            CCTCGTCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACAGACGCTCGCTGCCACTGGAATGTTGCATAATCCACCAGGCGTTCTGGCACCTCATCGCCATGTAGTACCAGGC  >  NZ_CP009273/2413273‑2413435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑