Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,426,064	A→G	S120P (TCC→CCC)	folC ←	bifunctional tetrahydrofolate synthase/dihydrofolate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,426,064	0	A	G	100.0%	67.5 / NA	19	S120P (TCC→CCC)	folC	bifunctional tetrahydrofolate synthase/dihydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/9);  total (10/9)

GCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACA  >  NZ_CP009273/2425977‑2426140                                                                                        |                                                                             gCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGgaa                                                                            <  1:270855/90‑1 (MQ=255) gCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGgaa                                                                            <  2:219940/90‑1 (MQ=255)              aaTCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGc                                                               <  1:154503/90‑1 (MQ=255)              aaTCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGc                                                               <  1:357058/90‑1 (MQ=255)              aaTCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGc                                                               <  1:385858/90‑1 (MQ=255)              aaTCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGc                                                               <  1:471521/90‑1 (MQ=255)                   ccacGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGAtt                                                          >  2:230465/1‑90 (MQ=255)                        tCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAAtc                                                     >  2:109898/1‑90 (MQ=255)                           aGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTcc                                                  <  2:28282/90‑1 (MQ=255)                                                   aaCGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGAt                          >  1:142702/1‑90 (MQ=255)                                                       ccGACAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCg                      >  1:79850/1‑90 (MQ=255)                                                        cGACAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGGGGGCCGATTCCgg                     >  2:414369/1‑90 (MQ=255)                                                          aCAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCa                   <  1:230465/90‑1 (MQ=255)                                                           cAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCaa                  <  1:47594/90‑1 (MQ=255)                                                           cAGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCaa                  <  2:470748/90‑1 (MQ=255)                                                            aGCGTACCGTACTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTTGGCCGATTTCGGCAAt                 >  2:212584/1‑90 (MQ=255)                                                               gtaccgtacTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTcc              >  2:59146/1‑90 (MQ=255)                                                                    gtacTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGcc         >  1:197386/1‑90 (MQ=255)                                                                     tacTCGAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGccc        >  1:255056/1‑90 (MQ=255)                                                                          gAAATAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGcac   <  1:446933/90‑1 (MQ=255)                                                                           aaaTAGGTCAGGGGAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGGCGATTCCGGCAATTCCTGGCCCTGcacc  >  2:112887/1‑89 (MQ=255)                                                                                        |                                                                             GCCCTACTTCCAGAATCACCACGTCAAGTTGTGCCTGCTTGAACAGCCACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACA  >  NZ_CP009273/2425977‑2426140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑