Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,432,392	A→G	I322V (ATT→GTT)	flk →	flagella biosynthesis regulator Flk

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,432,392	0	A	G	100.0%	65.4 / NA	19	I322V (ATT→GTT)	flk	flagella biosynthesis regulator Flk
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/6);  total (13/6)

AACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAG  >  NZ_CP009273/2432306‑2432472                                                                                       |                                                                                 aaCCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGGTTa                                                                               <  2:31336/90‑1 (MQ=255)     gctgctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGGTTATCCt                                                                           <  2:401676/90‑1 (MQ=255)       tgctTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGGTTATCCTGa                                                                         >  1:88865/1‑90 (MQ=255)                  tttCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGGTTATCCTGATGCTGGTctgg                                                              >  1:155761/1‑90 (MQ=255)                                                 tGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTa                               >  1:18972/1‑90 (MQ=255)                                                 tGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTa                               >  1:218330/1‑90 (MQ=255)                                                   gCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATc                             >  2:396930/1‑90 (MQ=255)                                                        gcgTCCCGCGCTGTGGATACTGTTAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGAc                        >  1:498018/1‑90 (MQ=255)                                                             ccGCGCTGTGGATACTGTTAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaataa                   <  1:193488/90‑1 (MQ=255)                                                             ccGCGCTGTGGATACTGTTAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaataa                   <  1:396930/90‑1 (MQ=255)                                                             ccGCGCTGTGGATACTGTTAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACaataa                   <  2:368239/90‑1 (MQ=255)                                                                  cTGTGGATACTGTTAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATAccc              <  1:293481/90‑1 (MQ=255)                                                                          aCTGTTAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGc      >  2:489062/1‑90 (MQ=255)                                                                              ttAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCagag  >  2:270115/1‑90 (MQ=255)                                                                              ttAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCagag  >  2:240523/1‑90 (MQ=255)                                                                              ttAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCagag  >  2:14934/1‑90 (MQ=255)                                                                              ttAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCagag  >  1:360541/1‑90 (MQ=255)                                                                              ttAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCagag  >  1:271900/1‑90 (MQ=255)                                                                              ttAGTCGCGGTTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCagag  >  1:157016/1‑90 (MQ=255)                                                                                       |                                                                                 AACCGCTGCTTAATCCTTTTCCACCGATGATGGACACGTTGCAAAATATGGCAACGCGTCCCGCGCTGTGGATACTGTTAGTCGCGATTATCCTGATGCTGGTCTGGCTGGTTCGTTAACCCCGACGAAATGACAGTATCGTGACAATAATACCCAGCACCGCAGAG  >  NZ_CP009273/2432306‑2432472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑