Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,485,462	A→G	intergenic (‑33/+21)	oxc ← / ← frc	oxalyl‑CoA decarboxylase/formyl‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,485,462	0	A	G	100.0%	78.1 / NA	22	intergenic (‑33/+21)	oxc/frc	oxalyl‑CoA decarboxylase/formyl‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/9);  total (13/9)

AATGCTTCAACGATGATATGCATACCATCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGAGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTGCAGCAATTTCATCGTCGCTATAACCCAGCT  >  NZ_CP009273/2485377‑2485538                                                                                      |                                                                             aaTGCTTCAACGATGATATGCATACCATCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCa                                                                          >  2:159325/1‑90 (MQ=255)        cAACGATGATATGCATACCATCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGccc                                                                   <  1:298871/90‑1 (MQ=255)         aaCGATGATATGCATACCATCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGcccc                                                                  <  1:18962/90‑1 (MQ=255)                       tACCATCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGAt                                                    >  2:389580/1‑90 (MQ=255)                       tACCATCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGAt                                                    <  1:159325/90‑1 (MQ=255)                           aTCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCg                                                >  2:251889/1‑90 (MQ=255)                           aTCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCg                                                >  1:269078/1‑90 (MQ=255)                            tCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGt                                               >  1:108622/1‑90 (MQ=255)                            tCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGt                                               >  2:22313/1‑90 (MQ=255)                            tCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGt                                               >  1:315326/1‑90 (MQ=255)                            tCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGt                                               >  1:108804/1‑90 (MQ=255)                             cTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTg                                              <  1:251889/90‑1 (MQ=255)                                       aaGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTc                                    >  1:503180/1‑90 (MQ=255)                                           tGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTg                                >  2:119955/1‑90 (MQ=255)                                                tGACATGATTAATAACCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTgcagca                           <  1:119955/90‑1 (MQ=255)                                                           ataaCCTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTGCAGCAATTTCAtcgtc                <  1:389580/90‑1 (MQ=255)                                                               ccTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTGCAGCAATTTCATCGTCGCta            <  2:108804/90‑1 (MQ=255)                                                                cTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTGCAGCAATTTCATCGTCGCtat           <  1:268743/90‑1 (MQ=255)                                                                cTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTGCAGCAATTTCATCGTCGCtat           >  2:171045/1‑90 (MQ=255)                                                                cTCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTGCAGCAATTTCATCGTCGCtat           >  2:241674/1‑90 (MQ=255)                                                                 tCGAAATTTTTATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTGCAGCAATTTCATCGTCGCtata          >  1:105451/1‑90 (MQ=255)                                                                         tttATAAATTGGGGGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTGCAGCAATTTCATCGTCGCTATAACCCAGCt  <  1:434962/90‑1 (MQ=255)                                                                                   ggggGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTGCAGCAATTTCATCGTCGCtat           <  2:270004/71‑1 (MQ=255)                                                                                      |                                                                             AATGCTTCAACGATGATATGCATACCATCTGTCATTTGAAGTTGATCTGACATGATTAATAACCTCGAAATTTTTATAAATTGGGAGCCAGAAGCCCCCGTTAATATCAGATGGCGTGGTTTTGCTTCATTGCAGCAATTTCATCGTCGCTATAACCCAGCT  >  NZ_CP009273/2485377‑2485538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑