Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,500,004	T→C	G36G (GGA→GGG)	ypdF ←	aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,500,004	0	T	C	100.0%	47.1 / NA	14	G36G (GGA→GGG)	ypdF	aminopeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/7);  total (7/7)

TTGCGCACGGGCTTCCACCTCAACGTAATAGCGCGAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGATCCGGTGGAGATCCCCAGATGCGGCTGTTTGTTCTGCCGTGAGGAGAGAAGCACTGCATCCAGTTGTTGC  >  NZ_CP009273/2499920‑2500073                                                                                     |                                                                      ttGCGCACGGGCTTCCACCTCAACGTAATAGCGCGAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGt                                                                  >  1:392048/1‑90 (MQ=255)   gcgcACGGGCTTCCACCTCAACGTAATAGCGCGAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGTgg                                                                >  1:283673/1‑90 (MQ=255)   gcgcACGGGCTTCCACCTCAACGTAATAGCGCGAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGTgg                                                                <  2:500214/90‑1 (MQ=255)    cgcACGGGCTTCCACCTCAACGTAATAGCGCGAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGTGga                                                               >  1:150509/1‑90 (MQ=255)                   cTCAACGTAATAGCGCGAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGTGGGGATCCCCAGATGCgg                                                <  1:184610/90‑1 (MQ=255)                    tCAACGTAATAGCGCGAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGTGGAGATCCCCAGATGCGGc                                               >  2:104014/1‑90 (MQ=255)                        cGTAATAGCGCAAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGTGGAGATCCCCAGATGCGGCtgtt                                           <  2:197604/90‑1 (MQ=255)                            aTAGCGCGAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGTGGAGATCCCCAGATGCGGCtgtttgtt                                       >  2:363854/1‑90 (MQ=255)                             tAGCGCGAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGTGGAGATCCCCAGATGCGGCTGTTTGTTc                                      <  2:252861/90‑1 (MQ=255)                                   gAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGTGGAGATCCCCAGATGCGGCTGTTTGTTCTGCCGt                                >  2:42616/1‑90 (MQ=255)                                            aGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGACCCGGTGGAGATCCCCAGATGCGGCTGTTTGTTCTGCCGTGAGgagaga                       <  1:363854/90‑1 (MQ=255)                                                               ggCTAATCACCACATAACCTGACCCGGTGGAGATCCCCAGATGCGGCTGTTTGTTCTGCCGTGAGGAGAGAAGCACTGCATCCAgttgtt    <  2:392048/90‑1 (MQ=255)                                                                gCTAATCACCACATAACCTGACCCGGTGGAGATCCCCAGATGCGGCTGTTTGTTCTGCCGTGAGGAGAGAAGCACTGCATCCAGttgttg   >  2:216375/1‑90 (MQ=255)                                                                 cTAATCACCACATAACCTGACCCGGTGGAGATCCCCAGATGCGGCTGTTTGTTCTGCCGTGAGGAGAGAAGCACTGCATCCAGTTGTTgc  <  1:272285/90‑1 (MQ=255)                                                                                     |                                                                      TTGCGCACGGGCTTCCACCTCAACGTAATAGCGCGAATCCACCAGAATGTGCGCACTTTCACGGCTAATCACCACATAACCTGATCCGGTGGAGATCCCCAGATGCGGCTGTTTGTTCTGCCGTGAGGAGAGAAGCACTGCATCCAGTTGTTGC  >  NZ_CP009273/2499920‑2500073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑