Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,557,422	T→C	D28D (GAT→GAC)	BW25113_RS12770 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,557,422	0	T	C	100.0%	27.9 / NA	9	D28D (GAT→GAC)	BW25113_RS12770	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/4);  total (5/4)

CATGAAAGTGTTAGGTAATATTTTATGGTGGGCTTTTGTTGGTTTCATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGATCCAGCTGTTGTGAACTATGAAGAGCCTTTGCCTATGGAGCTTGCCAACTA  >  NZ_CP009273/2557338‑2557472                                                                                     |                                                   cATGAAAGTGTTAGGTAATATTTTATGGTGGGCTTTTGTTGGTTTCATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGACCCAGc                                               <  1:196127/90‑1 (MQ=255)     aaaGTGTTAGGTAATATTTTATGGTGGGCTTTTGTTGGTTTCATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGACCCAGCtgtt                                           >  1:479049/1‑90 (MQ=255)      aaGTGTTAGGTAATATTTTATGGTGGGCTTTTGTTGGTTTCATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGACCCAGCtgttg                                          <  2:48442/90‑1 (MQ=255)                              gggCTTTTGTTGGTTTCATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGACCCAGCTGTTGTGAAc                                     <  1:330169/71‑1 (MQ=255)                              gggCTTTTGTTGGTTTCATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGACCCAGCTGTTGTGAAc                                     >  2:330169/1‑71 (MQ=255)                              gggCTTTTGTTGGTTTCATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGACCCAGCTGTTGTGAACTATGAAGAGCCTTTGCCTa                  >  1:354652/1‑90 (MQ=255)                              gggCTTTTGTTGGTTTCATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGACCCAGCTGTTGTGAACTATGAAGAGCCTTTGCCTa                  >  1:40226/1‑90 (MQ=255)                                          gTTTCATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGACCCAGCTGTTGTGAACTATGAAGAGCCTTTGCCTATGGAGCTTGCCa      <  1:423998/90‑1 (MQ=255)                                              cATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGACCCAGCTGTTGTGAACTATGAAGAGCCTTTGCCTATGGAGCTTGCCAACTa  >  1:252548/1‑90 (MQ=255)                                                                                     |                                                   CATGAAAGTGTTAGGTAATATTTTATGGTGGGCTTTTGTTGGTTTCATGGCTTACGCCACTTTGATTAAGCCGGACAACACTGATCCAGCTGTTGTGAACTATGAAGAGCCTTTGCCTATGGAGCTTGCCAACTA  >  NZ_CP009273/2557338‑2557472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑