Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	278,554	A→G	H189R (CAC→CGC)	yagE →	2‑keto‑3‑deoxygluconate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	278,554	0	A	G	100.0%	46.4 / NA	14	H189R (CAC→CGC)	yagE	2‑keto‑3‑deoxygluconate aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/3);  total (11/3)

ATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATAT  >  NZ_CP009273/278478‑278625                                                                             |                                                                        atCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGctc                                                            >  2:300257/1‑90 (MQ=255)   cGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTg                                                          >  1:378597/1‑90 (MQ=255)   cGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTg                                                          >  2:388383/1‑90 (MQ=255)      cATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCgg                                                       >  1:321930/1‑90 (MQ=255)        tCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCGGCt                                                     >  1:473367/1‑90 (MQ=255)               cacCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCGGCTAcgacga                                              <  2:19763/90‑1 (MQ=255)                    tCGACCCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCGGCTACGACGatcatc                                         <  2:350596/90‑1 (MQ=255)                         tCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTc                                    >  1:515035/1‑90 (MQ=255)                                       gcgcAGCATGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCGGCTACGACGatcatc                                         >  1:373187/1‑71 (MQ=255)                                       gcgcAGCATGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCGGCTACGACGatcatc                                         <  2:373187/71‑1 (MQ=255)                                            gcaTGATCCATACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTcggcg                 >  1:187313/1‑90 (MQ=255)                                                      tACCGTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGc       >  1:275837/1‑90 (MQ=255)                                                          gTCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGata   >  1:272250/1‑90 (MQ=255)                                                           tCAAAGGTGCCCATCCGCGCTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGatat  >  2:296311/1‑90 (MQ=255)                                                                             |                                                                        ATCGGCATCAAAGACACCATCGACTCCGTCGCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTCTGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATAT  >  NZ_CP009273/278478‑278625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑