Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,559,856	T→C	I130V (ATC→GTC)	eutA ←	ethanolamine ammonia‑lyase reactivating factor EutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,559,856	0	T	C	100.0%	49.5 / NA	15	I130V (ATC→GTC)	eutA	ethanolamine ammonia‑lyase reactivating factor EutA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/8);  total (7/8)

CACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAG  >  NZ_CP009273/2559778‑2559916                                                                               |                                                             ccccgccgATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGa                                                   <  1:405958/88‑1 (MQ=255)  aCCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAg                                                  >  2:21483/1‑90 (MQ=255)     gccgATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTg                                               <  2:151979/90‑1 (MQ=255)      ccgATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGc                                              <  2:329523/90‑1 (MQ=255)                tatTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGgcgc                                    <  1:424834/90‑1 (MQ=255)                     aGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGGCGCTGGCa                               <  1:179087/90‑1 (MQ=255)                           cGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACa                         >  2:465541/1‑90 (MQ=255)                                caGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATc                    >  1:309905/1‑90 (MQ=255)                                caGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATc                    >  2:230846/1‑90 (MQ=255)                                     gttgttCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCa               >  1:442966/1‑90 (MQ=255)                                      ttgttCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAg              <  1:21483/90‑1 (MQ=255)                                      ttgttCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAg              <  2:363451/90‑1 (MQ=255)                                                  ggTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAg              >  1:352303/1‑78 (MQ=255)                                                  ggTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAg              <  2:352303/78‑1 (MQ=255)                                                  ggTTTGTGCCCCGGCTCCGTGACCGGCGACCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGagagag  >  2:431018/1‑90 (MQ=255)                                                                               |                                                             CACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAG  >  NZ_CP009273/2559778‑2559916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑