| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,621,216 | A→G | I361T (ATC→ACC) | pdeF ← | cyclic‑guanylate‑specific phosphodiesterase PdeF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,621,216 | 0 | A | G | 100.0% | 58.0 / NA | 17 | I361T (ATC→ACC) | pdeF | cyclic‑guanylate‑specific phosphodiesterase PdeF |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (7/10); total (7/10) | |||||||||||
AAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTC > NZ_CP009273/2621129‑2621301 | aaGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTg < 1:356912/90‑1 (MQ=255) cAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTGCGTAAATAACaa > 1:224970/1‑90 (MQ=255) agaAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTGCGTAAATAACAAAgcgc < 1:384863/90‑1 (MQ=255) agaAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTGCGTAAATAACAAAgcgc < 1:195869/90‑1 (MQ=255) tttttGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTGCGTAAATAACAAAGCGCAGACCa < 1:367205/90‑1 (MQ=255) cGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAAcgcg > 2:508226/1‑90 (MQ=255) cGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAAcgcg < 2:133740/90‑1 (MQ=255) gcagcaTGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAAcgcgc > 1:366484/1‑90 (MQ=255) gcagcaTGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAAcgcgc > 2:258921/1‑90 (MQ=255) cagcaTGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAAcgcgcg < 2:421865/90‑1 (MQ=255) atgCCATAGTTCTTAACCAGCATTTCCATGCCAGGGGTGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAACGCGCGATTCAgg < 2:107409/90‑1 (MQ=255) aGCATTTCCATGCCAGGGGTGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGa > 1:458371/1‑90 (MQ=255) gCATTTCCATGCCAGGGGTGCGTAAATAACAAAgcgc < 2:218096/37‑1 (MQ=38) gCATTTCCATGCCAGGGGTGCGTAAATAACAAAgcgc > 1:218096/1‑37 (MQ=38) aTGCCAGGGGTGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAACGCGCGATTCTGGGCGCGAACATTTGGCAGATGAaccacc < 1:508226/90‑1 (MQ=255) cAGGGGTGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGt < 2:116249/90‑1 (MQ=255) aGGGGTGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTc > 1:430958/1‑90 (MQ=255) | AAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGCATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTC > NZ_CP009273/2621129‑2621301 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |