| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,649,892 | A→G | intergenic (‑175/+3) | pepB ← / ← iscX | aminopeptidase PepB/Fe‑S cluster assembly protein IscX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,649,892 | 0 | A | G | 78.6% | 23.2 / 3.1 | 14 | intergenic (‑175/+3) | pepB/iscX | aminopeptidase PepB/Fe‑S cluster assembly protein IscX |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (7/4); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGATTTTCTCGTTGGATGCCTGCGGGTCGTCGTCGAAA > NZ_CP009273/2649815‑2649973 | ctccAGGAGCATAGATAACTATGGGACTGGGGTGAATGAGCGCAGCCAACGCATCTGGGGCGTGAGGCATGACGCGGAAATTATTCGGcc < 2:372830/88‑1 (MQ=38)ccccAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGGAATTATTCGGcc < 1:32642/90‑1 (MQ=255) tATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGGAATTATTCGGCCTCGTCCAGCCAGACTAACa > 1:55611/1‑90 (MQ=255) caTGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGGAATTATTCGGCCTCGTCCAGCCAGACTAACa > 1:473007/2‑90 (MQ=255) aTGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGGAATTATTCGGCCTCGTCCAGCCAGACTAACaa > 1:236412/1‑90 (MQ=255) ggggTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGGAATTATTCGGCCTCGTCCAGCCAGAc > 1:485697/1‑76 (MQ=255) ggggTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGGAATTATTCGGCCTCGTCCAGCCAGAc < 2:485697/76‑1 (MQ=255) ggggTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGGAATTATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCtt > 2:11057/1‑90 (MQ=255) ggggTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCGTc > 1:311449/1‑67 (MQ=255) ggggTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCGTc < 2:311449/67‑1 (MQ=255) aCGCATCTGCGGCGTGAAGCATGACGCGGGAATTATTCGGCCTCGTCCAGCCAGACTAAc < 1:490852/60‑1 (MQ=255) aCGCATCTGCGGCGTGAAGCATGACGCGGGAATTATTCGGCCTCGTCCAGCCAGACTAAc > 2:490852/1‑60 (MQ=255) gTGAAGCATGACGCGGGAATTATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGATTTTCTCGTTGGATGCCTGCGGgtcgt < 2:23809/90‑1 (MQ=255) tGACGCGGGAATTATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGATTTTCTCGTTGGATGCCTGCGGGTCGTCGTCGaaa > 2:255774/1‑90 (MQ=255) | CCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAATTATTCGGCCTCGTCCAGCCAGACTAACAAAATCGCTTCGAGGATTTTCTCGTTGGATGCCTGCGGGTCGTCGTCGAAA > NZ_CP009273/2649815‑2649973 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |