Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,695,236	T→C	D198G (GAC→GGC)	recO ←	DNA repair protein RecO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,695,236	0	T	C	100.0%	40.6 / NA	13	D198G (GAC→GGC)	recO	DNA repair protein RecO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/6);  total (7/6)

CTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTG  >  NZ_CP009273/2695155‑2695308                                                                                  |                                                                         cTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAAt                                                                  >  1:100447/1‑90 (MQ=255)       tttAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGt                                                            <  2:11553/90‑1 (MQ=255)                         aGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAAc                                          <  1:71976/90‑1 (MQ=255)                          gATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAACt                                         <  1:159805/90‑1 (MQ=255)                                gCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTc                                   >  2:391647/1‑90 (MQ=255)                                          cATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGt                         >  1:219071/1‑90 (MQ=255)                                          cATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGt                         >  1:37051/1‑90 (MQ=255)                                          cATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGt                         >  1:510963/1‑90 (MQ=255)                                          cATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGt                         >  2:21684/1‑90 (MQ=255)                                               gggTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTAt                    >  1:164995/1‑90 (MQ=255)                                               gggTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTAt                    <  2:210288/90‑1 (MQ=255)                                                          ttCGCGGCGCGCAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAacg         <  2:480114/90‑1 (MQ=255)                                                                 cgcgcAGTGTGTCTGCGCCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTg  <  1:291987/90‑1 (MQ=255)                                                                                  |                                                                         CTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAGGAAATTCCCGTGCGTTTAACGCTTTTAACTGCCTTCCGGTGAACGTTTTATTGTCGATAACGACGCTTG  >  NZ_CP009273/2695155‑2695308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑