Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,734,303	A→G	T69T (ACT→ACC)	aroF ←	3‑deoxy‑7‑phosphoheptulonate synthase AroF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,734,303	0	A	G	100.0%	92.3 / NA	26	T69T (ACT→ACC)	aroF	3‑deoxy‑7‑phosphoheptulonate synthase AroF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/12);  total (14/12)

CGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTAC  >  NZ_CP009273/2734232‑2734388                                                                        |                                                                                      cgcATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATg                                                                     <  1:204828/90‑1 (MQ=255)        ccAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAg                                                              <  1:215021/90‑1 (MQ=255)         cAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAgg                                                             <  2:200155/90‑1 (MQ=255)           gATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGAc                                                           >  1:514918/1‑90 (MQ=255)            aTAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGAcc                                                          <  2:462815/90‑1 (MQ=255)             tAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACca                                                         >  1:133180/1‑90 (MQ=255)               gagGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACcaca                                                       >  2:7430/1‑90 (MQ=255)                agGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACAt                                                      <  1:254984/90‑1 (MQ=255)                 ggCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACAta                                                     >  1:505093/1‑90 (MQ=255)                 ggCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACAta                                                     >  1:426862/1‑90 (MQ=255)                         cTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACcagc                                             <  2:190765/90‑1 (MQ=255)                                ctGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAg                                      >  1:363202/1‑90 (MQ=255)                                ctGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAg                                      >  1:235540/1‑90 (MQ=255)                                       aaGGGCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATcgcg                               >  1:62672/1‑90 (MQ=255)                                          ggCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGccc                            <  1:370494/90‑1 (MQ=255)                                          ggCTTTAAATCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGccc                            <  1:31428/90‑1 (MQ=255)                                                 aaTCGACGAGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGata                     <  1:246093/90‑1 (MQ=255)                                                    cgacgaGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGataata                  <  2:426862/90‑1 (MQ=255)                                                    cgacgaGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGataata                  >  2:512289/1‑90 (MQ=255)                                                     gacgaGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACcagc                                             <  2:174931/62‑1 (MQ=255)                                                     gacgaGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACcagc                                             >  1:174931/1‑62 (MQ=255)                                                     gacgaGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAatat                 >  1:455222/1‑90 (MQ=255)                                                     gacgaGCATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAatat                 >  1:8360/1‑90 (MQ=255)                                                           cATATTCCAGAGCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGaaa           <  1:512289/90‑1 (MQ=255)                                                                   agagCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTa   >  1:248919/1‑90 (MQ=255)                                                                    gagCGGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTAc  >  2:443804/1‑90 (MQ=255)                                                                        |                                                                                      CGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCAGAGCAGTTTCCGGATCATGAATGGAACAAGGACCACATACTACCAGCAGACGAGGATCGCGCCCGGCGATAATATCTGAAATGCTTTTAC  >  NZ_CP009273/2734232‑2734388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑