| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,744,967 | A→G | I260V (ATT→GTT) | nadK → | NAD(+) kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,744,967 | 0 | A | G | 88.2% | 42.5 / ‑0.6 | 17 | I260V (ATT→GTT) | nadK | NAD(+) kinase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (5/10); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGATTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGT > NZ_CP009273/2744886‑2745051 | ttttCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGATTCGTCGc > 2:425943/1‑90 (MQ=255) aTCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGAtt < 2:338210/90‑1 (MQ=255) aTCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGATTCGTCGCTGTGAtt < 1:425943/90‑1 (MQ=255) gCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTAcc < 2:154170/90‑1 (MQ=255) ggAAATCAGTTGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGAt > 2:138273/1‑90 (MQ=255) cAGTTGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGATTCATcc < 1:6471/90‑1 (MQ=255) gTTGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGa < 1:361306/90‑1 (MQ=255) ttGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGaa < 1:244038/90‑1 (MQ=255) tGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGaaa > 1:357988/1‑90 (MQ=255) aCAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGAtt < 2:357988/90‑1 (MQ=255) aCTGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCaa < 1:412259/90‑1 (MQ=255) aCTGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCaa > 2:412259/1‑90 (MQ=255) tGCCGATTCAGGAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCAaca < 1:94800/90‑1 (MQ=255) ggAAGGTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCAACACATTAAGCAc < 2:517228/90‑1 (MQ=255) gTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGAtt < 2:218915/47‑1 (MQ=255) gTGAAGATGTCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGAtt > 1:218915/1‑47 (MQ=255) tCCTGGTTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGt > 1:171147/1‑90 (MQ=255) | TTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCACTGCCGATTCAGGAAGGTGAAGATGTCCTGATTCGTCGCTGTGATTACCATCTGAATCTGATTCATCCGAAAGATTACAGTTATTTCAACACATTAAGCACCAAGCTCGGCTGGT > NZ_CP009273/2744886‑2745051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |