Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,787,427	T→C	intergenic (+53/‑185)	gabT → / → gabP	4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,787,427	0	T	C	100.0%	35.0 / NA	11	intergenic (+53/‑185)	gabT/gabP	4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/8);  total (3/8)

TCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGC  >  NZ_CP009273/2787340‑2787496                                                                                        |                                                                      tcatcaGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGAccc                                                                     <  1:153259/90‑1 (MQ=255)           gtgtTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGACCCAGGTCGGATa                                                           <  1:416593/90‑1 (MQ=255)                     gaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGACCCAGGTCGGATAAg                                                         >  1:317232/1‑82 (MQ=255)                     gaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGACCCAGGTCGGATAAg                                                         <  2:317232/82‑1 (MQ=255)                     gaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGACCCAGGTCGGATAAGGCGTTTAc                                                 >  2:225505/1‑90 (MQ=255)                       ggCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGACCCAGGTCGGATAAGGCGTTTAcgc                                               <  2:393562/90‑1 (MQ=255)                                 tAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGACCCAGGTCGGATAAGGCGTTTACGCCGCATCCGAc                                     <  1:183537/90‑1 (MQ=255)                                       cgcTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGACCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTg                               >  2:480350/1‑90 (MQ=255)                                                     aGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGACCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa                 <  1:23442/90‑1 (MQ=255)                                                      ggCGACGCTGCGCGTCTTGTCCGGCCTACGGGGACCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaag                <  1:225505/90‑1 (MQ=255)                                                                    tCTTGTCCGGCCTACGGGGACCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGc  <  1:413781/90‑1 (MQ=255)                                                                    tCTTGTCCGGCCTACGGGGACCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGc  <  2:365969/90‑1 (MQ=255)                                                                                        |                                                                      TCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGC  >  NZ_CP009273/2787340‑2787496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑