Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,795,453	C→T	G249G (GGC→GGT)	nrdE →	class 1b ribonucleoside‑diphosphate reductase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,795,453	0	C	T	100.0%	39.2 / NA	12	G249G (GGC→GGT)	nrdE	class 1b ribonucleoside‑diphosphate reductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/9);  total (3/9)

AATCTTCTGGCGTAATTCCGGTGATGAAAATGCTGGAAGACGCATTTTCCTATGCCAACCAACTCGGCGCTCGTCAGGGGGCTGGTGCAGTCTATTTACATGCTCATCATCCCGATATTCTGCGTTTTCTCGACACGAAACGGGAA  >  NZ_CP009273/2795386‑2795531                                                                    |                                                                               actctgctGGAGTAATTCCGGTGATGAAAATGCTGGAAGACGCATTTTCCTTTGCCAACCAACTCGGTGCTCGTCAGGGGGCTGGTGCAg                                                          <  2:248068/88‑1 (MQ=255) aatcTTCTGGCGTAATTCCGGTGATGAAAATGCTGGAAGACGCATTTTCCTATGCCAACCAACTCGGTGCTCGTCAGGGGGCTGGTGCAg                                                          <  1:17448/90‑1 (MQ=255) aatcTTCTGGCGTAATTCCGGTGATGAAAATGCTGGAAGACGCATTTTCCTATGCCAACCAACTCGGTGCTCGTCAGGGGGCTGGTGCAg                                                          <  2:284668/90‑1 (MQ=255) aatcTTCTGGCGTAATTCCGGTGATGAAAATGCTGGAAGACGCATTTTCCTATGCCAACCAACTCGGTGCTCGTCAGGGGGCTGGTGCAg                                                          <  2:287326/90‑1 (MQ=255) aatcTTCTGGCGTAATTCCGGTGATGAAAATGCTGGAAGACGCATTTTCCTATGCCAACCAACTCGGTGCTCGCCAGGGGGCTGGTGCAg                                                          <  1:450700/90‑1 (MQ=255)   tcttctGGCGTAATTCCGGTGATGAAAATGCTGGAAGACGCATTTTCCTATGCCAACCAACTCGGTGCTCGTCAGGGGGCTGGTGCAGTc                                                        <  1:511920/90‑1 (MQ=255)                   cGGTGATGAAAATGCTGGAAGACGCATTTTCCTATGCCAACCAACTCGGTGCTCGTCAGGGGGCTGGTGCAGTCTATTTACATGCtcatc                                        <  2:216432/90‑1 (MQ=255)                        atgaAAATGCTGGAAGACGCATTTTCCTATGCCAACCAACTCGGTGCTCGTCAGGGGGCTGGTGc                                                            >  1:296138/1‑65 (MQ=255)                        atgaAAATGCTGGAAGACGCATTTTCCTATGCCAACCAACTCGGTGCTCGTCAGGGGGCTGGTGc                                                            <  2:296138/65‑1 (MQ=255)                                                         aaccaacTCGGTGCTCGTCAGGGGGCTGGTGCAGTc                                                        >  1:413336/1‑36 (MQ=38)                                                         aaccaacTCGGTGCTCGTCAGGGGGCTGGTGCAGTc                                                        <  2:413336/36‑1 (MQ=38)                                                         aaccaacTCGGTGCTCGTCAGGGGGCTGGTGCAGTCTATTTACATGCTCATCATCCCGATATTCTGCGTTTTCTCGACACGAAACGGGaa  >  1:377444/1‑90 (MQ=255)                                                                    |                                                                               AATCTTCTGGCGTAATTCCGGTGATGAAAATGCTGGAAGACGCATTTTCCTATGCCAACCAACTCGGCGCTCGTCAGGGGGCTGGTGCAGTCTATTTACATGCTCATCATCCCGATATTCTGCGTTTTCTCGACACGAAACGGGAA  >  NZ_CP009273/2795386‑2795531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑