| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 302,805 | T→C | M747V (ATG→GTG) | ecpC ← | fimbrial usher EcpC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 302,805 | 0 | T | C | 93.3% | 41.2 / ‑3.2 | 15 | M747V (ATG→GTG) | ecpC | fimbrial usher EcpC |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (6/8); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01 | |||||||||||
GGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCAATGACAGCGACATTGCCTGGATAGAGAGTCAGACGACTTTTGCGGCCGCTGACGATATCGTA > NZ_CP009273/302727‑302886 | ggCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCACCTGCTTCACct > 2:420026/1‑90 (MQ=255) ccGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCACCTGCTTCACctct < 2:233834/90‑1 (MQ=255) tAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCACCTGCTTCACCTCTGGCTCAATGACAg < 2:392829/90‑1 (MQ=255) cGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCACCTGCTTCACCTCTGGCTCAATGACAGCGACa < 1:8143/90‑1 (MQ=255) cGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATTCGACCGGAGACGGTAACGACCTGCTTCACCTCTGGCTCAATGACAGCGACATTGc < 1:28004/90‑1 (MQ=255) cGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCACCTGCTTCACCTCTGGCTCAATGACAGCGACATTGc < 1:177378/90‑1 (MQ=255) cagcagTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCACCTGCTTCACCTCTGGCTCAATGACAGCGACa > 1:402853/1‑79 (MQ=255) cagcagTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCACCTGCTTCACCTCTGGCTCAATGACAGCGACa < 2:402853/79‑1 (MQ=255) cTTCCGCACGGATACGACCGGAGACGGTAACCACCTGCTTCACCTCTGGCTCAATGACAGCGACATTGCCTGGATAGAGAGTCAgacgac > 2:125481/1‑90 (MQ=255) ccGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCAATGACAGCGACATTGCCTGGATAGAGAGTCAGACGACttt > 1:252906/1‑90 (MQ=255) ccGCACGGATACGACCGGAGACGGTAACCACCTGCTTCACCTCTGGCTCAATGACAGCGACATTGCCTGGATAGAGAGTCAGACGACttt > 2:349165/1‑90 (MQ=255) cACGGATACGACCGGAGACGGTAACCACCTGCTTCACCTCTGGCTCAATGACAGCGACATTGCCTGGATAGAGAGTCAGACGACTTTTGc < 2:392258/90‑1 (MQ=255) ggAGACGGTAACCACCTGCTTCACCTCTGGCTCAATGACAGCGACATTGCCTGGATAGAGAGTCAGACGACTTTTGCGGCCGCTGACGat > 2:394410/1‑90 (MQ=255) gagaCGGTAACCACCTGCTTCACCTCTGGCTCAATGACAGCGACATTGCCTGGATAGAGAGTCAGACGACTTTTGCGGCCGCTGACGata > 1:107850/1‑90 (MQ=255) ggTAACCACCTGCTTCACCTCTGGCTCAATGACAGCGACATTGCCTGGATAGAGAGTCAGACGACTTTTGCGGCCGCTGACGATATCGTa < 1:123528/90‑1 (MQ=255) | GGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCAATGACAGCGACATTGCCTGGATAGAGAGTCAGACGACTTTTGCGGCCGCTGACGATATCGTA > NZ_CP009273/302727‑302886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |