Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,834,040	A→G	K386K (AAA→AAG)	ascF →	PTS cellobiose/arbutin/salicin transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,834,040	0	A	G	100.0%	23.1 / NA	8	K386K (AAA→AAG)	ascF	PTS cellobiose/arbutin/salicin transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/6);  total (2/6)

TCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCC  >  NZ_CP009273/2833956‑2834113                                                                                     |                                                                          tCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAGCGTcc                                                                      <  1:186561/90‑1 (MQ=255)     aCGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAGCGTCCGCtt                                                                  <  1:83241/90‑1 (MQ=255)          gctggctgCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAGCGTCCGCTTATCGc                                                             <  2:412951/90‑1 (MQ=255)                                        ggggATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAGCGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGgcgc                               <  1:487496/90‑1 (MQ=255)                                        ggggATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAGCGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGgcgc                               <  1:5799/90‑1 (MQ=255)                                                    aCCGGCGTTATATGGCGTGGCGATCCGCCTGAAGCGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTAt                   >  2:156212/1‑90 (MQ=255)                                                                  gcgtggcgATCCGCCTGAAGCGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGGGTgccc     >  1:16587/1‑89 (MQ=255)                                                                     tggcgATCCGCCTGAAGCGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTgccagcc  <  2:492187/90‑1 (MQ=255)                                                                                     |                                                                          TCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCC  >  NZ_CP009273/2833956‑2834113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑