Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,851,528	T→C	T359T (ACT→ACC)	mutS →	DNA mismatch repair protein MutS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,851,528	0	T	C	100.0%	48.5 / NA	15	T359T (ACT→ACC)	mutS	DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

GGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAG  >  NZ_CP009273/2851471‑2851611                                                          |                                                                                    ggTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCa                                                     >  1:439892/1‑90 (MQ=255)  gTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCAc                                                    <  1:12322/90‑1 (MQ=255)       gCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCttt                                               >  2:24875/1‑90 (MQ=255)         gTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATgcgc                                                       <  1:2448/80‑1 (MQ=255)         gTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATgcgc                                                       >  2:2448/1‑80 (MQ=255)            aGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCAcg                                                                          <  1:322821/58‑1 (MQ=255)            aGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCAcg                                                                          >  2:322821/1‑58 (MQ=255)            aGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc                                          >  1:3229/1‑90 (MQ=255)            aGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc                                          >  2:342369/1‑90 (MQ=255)              gTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCaa                                        <  2:503884/90‑1 (MQ=255)                               tATTCTGGCACGTCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgtgc                       >  1:13082/1‑90 (MQ=255)                                 ttCTGGCACGTCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTgcgc                     <  2:3229/90‑1 (MQ=255)                                           tCTGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAAc           <  1:259054/90‑1 (MQ=255)                                             tGGCTTTACGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTg         <  1:96307/90‑1 (MQ=255)                                                    aCGAACCGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAg  >  1:7529/1‑90 (MQ=255)                                                          |                                                                                    GGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAG  >  NZ_CP009273/2851471‑2851611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑