Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,862,116 T→C intergenic (‑4/+136) nlpD ← / ← pcm murein hydrolase activator NlpD/protein‑L‑isoaspartate O‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,862,1160TC100.0% 67.3 / NA 20intergenic (‑4/+136)nlpD/pcmmurein hydrolase activator NlpD/protein‑L‑isoaspartate O‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/14);  total (6/14)

GTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACG  >  NZ_CP009273/2862029‑2862197
                                                                                       |                                                                                 
gtCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTAccc                                                                                 >  1:262438/1‑90 (MQ=255)
        aGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGaa                                                                         >  1:521146/1‑90 (MQ=255)
         gCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGaaa                                                                        >  1:432050/1‑90 (MQ=255)
          ccTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGaaaa                                                                       >  2:33851/1‑90 (MQ=255)
              ccagccaTAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTg                                                                   <  1:26568/90‑1 (MQ=255)
                            aCCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTg                                                     <  2:521146/90‑1 (MQ=255)
                            aCCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTg                                                     <  2:380544/90‑1 (MQ=255)
                            aCCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTg                                                     <  1:520371/90‑1 (MQ=255)
                              cAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGAc                                                   <  1:425193/90‑1 (MQ=255)
                                      aaGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTAc                                           >  1:195857/1‑90 (MQ=255)
                                      aaGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTAc                                           <  2:43972/90‑1 (MQ=255)
                                       aGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAAcc                                                         >  2:54607/1‑75 (MQ=255)
                                       aGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAAcc                                                         <  1:54607/75‑1 (MQ=255)
                                               tGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAgg                                  <  1:381910/90‑1 (MQ=255)
                                                  ggcgAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCAc                               <  1:42655/90‑1 (MQ=255)
                                                  ggcgAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCAc                               <  1:420379/90‑1 (MQ=255)
                                                  ggcgAACGGTGAATTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCAc                               <  1:179676/90‑1 (MQ=255)
                                                             aaTTTTGGGCTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAaca                    <  2:432050/90‑1 (MQ=255)
                                                                      cTTCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAg           >  1:129520/1‑90 (MQ=255)
                                                                       ttCCCGCGCTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGa          <  1:206146/90‑1 (MQ=255)
                                                                               cTCATTTACCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACg  <  2:430312/90‑1 (MQ=255)
                                                                                       |                                                                                 
GTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGACAAAGCCGCAATGCGGCGAACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACG  >  NZ_CP009273/2862029‑2862197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: