Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,940,916	T→C	noncoding (61/77 nt)	BW25113_RS14640 →	tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,940,916	0	T	C	100.0%	71.2 / NA	21	noncoding (61/77 nt)	BW25113_RS14640	tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

AGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGG  >  NZ_CP009273/2940846‑2940996                                                                       |                                                                                 aGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATc                                                               <  1:329840/90‑1 (MQ=14)           cgcgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTtgatg                                                     <  1:262720/90‑1 (MQ=255)           cgcgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTtgatg                                                     <  1:316563/90‑1 (MQ=255)            gcgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTtgat                                                      <  1:295073/88‑1 (MQ=255)            gcgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTtgat                                                      >  2:295073/1‑88 (MQ=255)                     agcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGc                                           <  2:163915/90‑1 (MQ=255)                        agcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGt                                        <  2:140718/90‑1 (MQ=255)                         gcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTtgatg                                                     <  1:318566/76‑1 (MQ=255)                         gcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTtgatg                                                     >  2:318566/1‑76 (MQ=255)                         gcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGGAGTa                                       >  2:476253/1‑90 (MQ=255)                                   tcgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTtgat                                                      >  1:40680/1‑65 (MQ=255)                                   tcgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTtgat                                                      <  2:40680/65‑1 (MQ=255)                                   tcgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGAcgcg                             >  2:147920/1‑90 (MQ=255)                                            tCATAACCCGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGagc                    <  1:226922/90‑1 (MQ=255)                                                    cGAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGt            >  1:328899/1‑90 (MQ=255)                                                     gAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGagcag                  <  1:40823/83‑1 (MQ=255)                                                     gAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGagcag                  >  2:40823/1‑83 (MQ=255)                                                     gAAGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTa           >  2:218036/1‑90 (MQ=255)                                                       aGGTCGTCGGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGc         >  1:434014/1‑90 (MQ=255)                                                            gtcgGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtc    >  2:373291/1‑90 (MQ=255)                                                              cgGTTCAAACCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCgg  <  1:61353/90‑1 (MQ=255)                                                                       |                                                                                 AGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGG  >  NZ_CP009273/2940846‑2940996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑