Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,000,381	T→G	V253G (GTC→GGC)	ygeW →	knotted carbamoyltransferase YgeW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,000,381	0	T	G	100.0%	74.2 / NA	21	V253G (GTC→GGC)	ygeW	knotted carbamoyltransferase YgeW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/13);  total (8/13)

CATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGT  >  NZ_CP009273/3000296‑3000463                                                                                      |                                                                                   cATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCAcc                                                                                <  1:147726/90‑1 (MQ=255)  aTCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCa                                                                               >  2:180633/1‑90 (MQ=255)                    tGATCCCGGATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCa                                                             >  1:146426/1‑90 (MQ=255)                     gATCCCGGATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCaa                                                            >  2:80893/1‑90 (MQ=255)                           ggATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGc                                                      >  2:332853/1‑90 (MQ=255)                               gtgGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGAc                                                  >  2:189290/1‑90 (MQ=255)                                 ggTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACAt                                                >  1:191805/1‑90 (MQ=255)                                            cGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGa                                     <  2:303058/90‑1 (MQ=255)                                            cGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGa                                     <  1:494391/90‑1 (MQ=255)                                                      tGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGc                           <  2:411725/90‑1 (MQ=255)                                                       gCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCa                          <  1:294484/90‑1 (MQ=255)                                                       gCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCa                          <  1:429037/90‑1 (MQ=255)                                                       gCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCa                          <  2:349486/90‑1 (MQ=255)                                                       gCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCa                          <  2:45729/90‑1 (MQ=255)                                                                         aGCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGaa        >  1:436607/1‑90 (MQ=255)                                                                          gCTTCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAag       <  2:262341/90‑1 (MQ=255)                                                                            ttCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAagag     <  2:327883/90‑1 (MQ=255)                                                                            ttCCGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAagag     <  2:503351/90‑1 (MQ=255)                                                                              ccGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCg   >  2:139437/1‑90 (MQ=255)                                                                               cGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGt  <  2:208008/90‑1 (MQ=255)                                                                               cGTCAGGGCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGt  <  2:381147/90‑1 (MQ=255)                                                                                      |                                                                                   CATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGT  >  NZ_CP009273/3000296‑3000463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑