| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,014,062 | A→G | D55G (GAC→GGC) | ygfM → | molybdopterin‑dependent oxidoreductase FAD‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,014,062 | 0 | A | G | 93.3% | 43.1 / ‑3.7 | 15 | D55G (GAC→GGC) | ygfM | molybdopterin‑dependent oxidoreductase FAD‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (9/5); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
GGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTA > NZ_CP009273/3013989‑3014139 | ggCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGGCTGGGTTGACTGGGAt < 1:144867/90‑1 (MQ=255) acacCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGAtt > 1:17883/1‑90 (MQ=255) cacCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCACTGCGGATTg > 1:398782/1‑90 (MQ=255) aaCCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCACTGCGGATTGgcgc > 1:208827/1‑90 (MQ=255) aaCCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCACTGCGGATTGgcgc > 2:36945/1‑90 (MQ=255) gTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGt > 1:220558/1‑90 (MQ=255) tAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCAc < 1:31364/65‑1 (MQ=255) tAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCAc > 2:31364/1‑65 (MQ=255) aGATTGCCATTTCCTTACAGGATCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGc < 1:493907/90‑1 (MQ=255) tttCCTTACAGGATCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTg > 2:424123/1‑90 (MQ=255) ttCCTTACAGGATCTGGAACTGGGCTGGGTTGACt < 1:253471/35‑1 (MQ=37) ttCCTTACAGGATCTGGAACTGGGCTGGGTTGACt > 2:253471/1‑35 (MQ=37) tACAGGATCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATgcgc < 1:424123/90‑1 (MQ=255) aTCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTa > 2:199777/1‑90 (MQ=255) aTCTGGAACTGGGCTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTa > 2:364266/1‑90 (MQ=255) | GGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTA > NZ_CP009273/3013989‑3014139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |