| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,035,080 | C→T | P137S (CCT→TCT) | ygfZ → | tRNA‑modifying protein YgfZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,035,080 | 0 | C | T | 91.7% | 36.9 / ‑6.4 | 12 | P137S (CCT→TCT) | ygfZ | tRNA‑modifying protein YgfZ |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (2/9); total (3/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTA > NZ_CP009273/3034993‑3035163 | gccATCGCGCCAGACGACGAGCGGGAGCTGCTTGGAGTTGCCGGTTTTCAGGCGCGGGCCGCGCTGGCAAATCTCTTTAGCGAACTTCCt > 2:256542/2‑90 (MQ=255) cgcgCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGTCTTCgaa < 1:20504/90‑1 (MQ=255) gacgaGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGTCTTCGAAAGAAAAACAg < 2:87494/90‑1 (MQ=255) gtgtGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGTCTTCGAAAGAAAAACAGGTAGTCa > 2:2905/1‑90 (MQ=255) tGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGTCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCAc < 2:379388/90‑1 (MQ=255) ccGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGTCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACtt < 2:15751/90‑1 (MQ=255) ttttCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGTCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCt > 2:40204/1‑90 (MQ=255) tttCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGTCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTa < 1:25/90‑1 (MQ=255) tttCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGTCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTa < 1:2905/90‑1 (MQ=255) tttCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGTCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTa < 1:515542/90‑1 (MQ=255) tttCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGTCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTa < 2:388887/90‑1 (MQ=255) gAACTGTCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTa < 2:318205/90‑1 (MQ=255) | ACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTA > NZ_CP009273/3034993‑3035163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |