Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,097,142	T→G	G257G (GGT→GGG)	mutY →	A/G‑specific adenine glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,097,142	0	T	G	100.0%	22.6 / NA	8	G257G (GGT→GGG)	mutY	A/G‑specific adenine glycosylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/1);  total (7/1)

ATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGC  >  NZ_CP009273/3097082‑3097227                                                             |                                                                                      aTTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGGTTATACTGTTTCCCGCAGTTTGCcgacga                                                          >  2:315771/1‑90 (MQ=255)        cACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGGTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGt                                                   >  1:245614/1‑90 (MQ=255)           gAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGGTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTg                                                >  1:322616/1‑90 (MQ=255)                           tGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGGTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGgcgc                                >  1:434579/1‑90 (MQ=255)                                      ccgccgAGCGGATTGTGGGGCGGGTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGAtt                     <  1:315771/90‑1 (MQ=255)                                      ccgccgAGCGGATTGTGGGGCGGGTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGAtt                     >  2:183332/1‑90 (MQ=255)                                      ccgccgAGCGGATTGTGGGGCGGGTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGAtt                     >  2:250320/1‑90 (MQ=255)                                                         gCGGGTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGc  >  2:442846/1‑90 (MQ=255)                                                             |                                                                                      ATTACAGCACGAAGATGAAGTATTGCTGGCGCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGC  >  NZ_CP009273/3097082‑3097227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑