Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	328,409	C→T	P110S (CCT→TCT)	pdeL →	DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	328,409	0	C	T	100.0%	34.5 / NA	10	P110S (CCT→TCT)	pdeL	DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/0);  total (10/0)

GTCACAGATATATTAATGATAATCACTATCACCATATCGTCACGCCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTGGATCCAACCGGTTTTCTGCGCGCAGACTGGCGTA  >  NZ_CP009273/328365‑328489                                             |                                                                                 gTCACAGATATATTAATGATAATCACTATCACCATATCGTCACGTCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGt                                     >  1:284577/1‑90 (MQ=255)   cacaGATATATTAATGATAATCACTATCACCATATCGTCACGTCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTgg                                   >  1:399394/1‑90 (MQ=255)   cacaGATATATTAATGATAATCACTATCACCATATCGTCACGTCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTgg                                   >  2:450886/1‑90 (MQ=255)            atTAATGATAATCACTATCACCATATCGTCACGTCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTGGATCCAACCg                          >  1:247264/1‑90 (MQ=255)               aaTGATAATCACTATCACCATATCGTCACGTCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTGGATCCAACCGGtt                       >  1:306693/1‑90 (MQ=255)                    tAATCACTATCACCATATCGTCACGTCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTGGATCCAACCGGTTTTCTg                  >  1:224478/1‑90 (MQ=255)                                  atatCGTCACGTCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTGGATCCAACCGGTTTTCTGCGCGCAGACTGGCg    >  2:335581/1‑90 (MQ=255)                                    atCGTCACGTCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTGGATCCAACCGGTTTTCTGCGCGCAGACTGGCGTa  >  1:310626/1‑90 (MQ=255)                                    atCGTCACGTCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTGGATCCAACCGGTTTTCTGCGCGCAGACTGGCGTa  >  1:350084/1‑90 (MQ=255)                                    atCGTCACGTCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTGGATCCAACCGGTTTTCTGCGCGCAGACTGGCGTa  >  1:42746/1‑90 (MQ=255)                                             |                                                                                 GTCACAGATATATTAATGATAATCACTATCACCATATCGTCACGCCTGAAGCCATCAGTCTGGCGTTGGAAAACCACGAATTCAAACCGTGGATCCAACCGGTTTTCTGCGCGCAGACTGGCGTA  >  NZ_CP009273/328365‑328489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑