Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,335,202	A→C	H193P (CAC→CCC)	kdsD →	arabinose‑5‑phosphate isomerase KdsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,335,202	0	A	C	100.0%	122.1 / NA	34	H193P (CAC→CCC)	kdsD	arabinose‑5‑phosphate isomerase KdsD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/13);  total (21/13)

TGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAA  >  NZ_CP009273/3335127‑3335286                                                                            |                                                                                     tGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTCACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCAc                                                                        <  1:75971/90‑1 (MQ=255)           cGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaa                                                              <  1:234430/90‑1 (MQ=255)            gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaaa                                                             >  2:304864/1‑90 (MQ=255)            gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaaa                                                             >  1:213158/1‑90 (MQ=255)            gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaaa                                                             >  2:283848/1‑90 (MQ=255)            gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaaa                                                             >  2:394724/1‑90 (MQ=255)            gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaaa                                                             >  2:140869/1‑90 (MQ=255)            gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaaa                                                             >  2:405191/1‑90 (MQ=255)            gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaaa                                                             >  1:440670/1‑90 (MQ=255)            gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaaa                                                             >  1:71229/1‑90 (MQ=255)            gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaa                                                              <  1:172545/89‑1 (MQ=255)            gATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTaa                                                              >  2:172545/1‑89 (MQ=255)                   tCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTctgc                                                      >  2:351610/1‑90 (MQ=255)                         tCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGcgcg                                                <  1:304864/90‑1 (MQ=255)                         tCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGcgcg                                                >  1:215532/1‑90 (MQ=255)                            cgcTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTaa                                             <  2:300933/90‑1 (MQ=255)                              cTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAAc                                           <  1:86003/90‑1 (MQ=255)                              cTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAAc                                           <  1:3480/90‑1 (MQ=255)                              cTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAAc                                           <  2:215532/90‑1 (MQ=255)                                gTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGa                                         >  1:499585/1‑90 (MQ=255)                                     aaGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGAtatta                                    <  2:71229/90‑1 (MQ=255)                                          cgcgGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTg                                                                      <  1:80648/51‑1 (MQ=255)                                          cgcgGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTg                                                                      >  2:80648/1‑51 (MQ=255)                                          cgcgGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCAt                               >  1:208451/1‑90 (MQ=255)                                          cgcgGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCAt                               >  1:327124/1‑90 (MQ=255)                                                     tgctgAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATga                    <  2:440670/90‑1 (MQ=255)                                                      gctgAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAAGTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATgag                   >  1:394234/1‑90 (MQ=255)                                                       ctgAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATgaga                  <  1:405191/90‑1 (MQ=255)                                                        tgAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGAt                 >  2:347477/1‑90 (MQ=255)                                                        tgAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGAt                 >  2:253658/1‑90 (MQ=255)                                                           aGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATccc              >  2:503952/1‑90 (MQ=255)                                                            gATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCg             <  2:98254/90‑1 (MQ=255)                                                                ttGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATg         >  2:356697/1‑90 (MQ=255)                                                                       ctcACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGaa  >  2:375895/1‑90 (MQ=255)                                                                            |                                                                                     TGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAA  >  NZ_CP009273/3335127‑3335286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑