Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,355,069	A→G	T179A (ACC→GCC)	gltF →	DUF1120 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,355,069	0	A	G	100.0%	92.2 / NA	26	T179A (ACC→GCC)	gltF	DUF1120 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/10);  total (16/10)

GACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCA  >  NZ_CP009273/3354982‑3355152                                                                                        |                                                                                    gACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGcc                                                                                   >  1:45612/1‑90 (MQ=255) gACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGcc                                                                                   >  2:462617/1‑90 (MQ=255)     ggTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCca                                                                               >  2:98935/1‑90 (MQ=255)     ggTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCca                                                                               <  1:6475/90‑1 (MQ=255)        cTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACAt                                                                            >  2:360190/1‑90 (MQ=255)            tggtggGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGaa                                                                        <  2:372027/90‑1 (MQ=255)               tggGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGc                                                                     >  1:56725/1‑90 (MQ=255)                gggATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGcc                                                                    >  1:9241/1‑90 (MQ=255)                 ggATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCt                                                                   <  2:56725/90‑1 (MQ=255)                          cggcggACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATg                                                          >  2:78640/1‑90 (MQ=255)                            gcggACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGgc                                                        >  2:511031/1‑90 (MQ=255)                            gcggACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGgc                                                        >  1:523014/1‑90 (MQ=255)                                       aGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTc                                             <  1:462617/90‑1 (MQ=255)                                           gAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTg                                         >  2:255536/1‑90 (MQ=255)                                             aaTTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGAc                                       >  2:348038/1‑90 (MQ=255)                                             aaTTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGAc                                       >  1:499400/1‑90 (MQ=255)                                                    gTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGc                                <  1:348038/90‑1 (MQ=255)                                                                 aaaCTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAgc                   <  1:98935/90‑1 (MQ=255)                                                                 aaaCTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAgc                   <  2:72844/90‑1 (MQ=255)                                                                   aCTGACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAgcgc                 <  1:360190/90‑1 (MQ=255)                                                                      gACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGt              >  2:224794/1‑90 (MQ=255)                                                                      gACCACTGGTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGt              >  1:26910/1‑90 (MQ=255)                                                                             ggTCAGCAGCGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTg       <  1:170926/90‑1 (MQ=255)                                                                                  gcagcGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCa  >  2:328088/1‑90 (MQ=255)                                                                                  gcagcGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCa  >  2:328709/1‑90 (MQ=255)                                                                                  gcagcGGCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCa  <  2:523014/90‑1 (MQ=255)                                                                                        |                                                                                    GACAGGTCTAATGGTGGGATTCACGCGGCGGACGATACAGGTGAAATTCCTGTCGACCTGATTCAAACTGACCACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCA  >  NZ_CP009273/3354982‑3355152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑