Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,471,902	A→G	intergenic (‑46/+49)	slyD ← / ← yheV	peptidylprolyl isomerase/YheV family putative metal‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,471,902	0	A	G	100.0%	88.9 / NA	25	intergenic (‑46/+49)	slyD/yheV	peptidylprolyl isomerase/YheV family putative metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/11);  total (14/11)

GATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCAT  >  NZ_CP009273/3471817‑3471985                                                                                      |                                                                                    gATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTc                                                                                 >  1:322344/1‑90 (MQ=255)    aGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAGGAATAGTGGCGCAGATTGTAGCGGGATTCTGc                                                                              <  2:114351/90‑1 (MQ=255)    aGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGc                                                                              <  2:96381/90‑1 (MQ=255)         aGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCg                                                                         <  1:102075/90‑1 (MQ=255)           gCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCg                                                                         <  2:384649/88‑1 (MQ=255)           gCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCg                                                                         >  1:384649/1‑88 (MQ=255)              gACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTAc                                                                    <  1:339852/90‑1 (MQ=255)              gACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTAc                                                                    <  1:340327/90‑1 (MQ=255)               accaccAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACt                                                                   >  1:488278/1‑90 (MQ=255)               accaccAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACt                                                                   >  2:252434/1‑90 (MQ=255)                  accaGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACTCAc                                                                <  2:81071/90‑1 (MQ=255)                        tCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCtt                                                          >  2:302888/1‑90 (MQ=255)                         cTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGc                                                                              <  2:257722/69‑1 (MQ=255)                         cTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGc                                                                              >  1:257722/1‑69 (MQ=255)                           tttGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTaaa                                                       >  2:504888/1‑90 (MQ=255)                                         gATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCat                                         <  2:465062/90‑1 (MQ=255)                                                   gAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTc                               >  1:35519/1‑90 (MQ=255)                                                       aTGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGa                           <  2:183065/90‑1 (MQ=255)                                                        tGGGAAGAATAGTGGCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGAt                          >  1:172780/1‑90 (MQ=255)                                                                     ggCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGAt             >  1:469977/1‑90 (MQ=255)                                                                     ggCGCAGATTGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGAt             >  1:270281/1‑90 (MQ=255)                                                                       cgcAGATTGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCa           <  2:403668/90‑1 (MQ=255)                                                                        gcAGATTGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCAc          >  1:424724/1‑90 (MQ=255)                                                                             ttGTAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCt     >  1:264278/1‑90 (MQ=255)                                                                                tAGCGGGATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCAt  >  2:443377/1‑90 (MQ=255)                                                                                      |                                                                                    GATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCAT  >  NZ_CP009273/3471817‑3471985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑