Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,504,746	G→A	G12G (GGC→GGT)	yhfY ←	PRD domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,504,746	0	G	A	100.0%	56.9 / NA	17	G12G (GGC→GGT)	yhfY	PRD domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/8);  total (9/8)

ACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCA  >  NZ_CP009273/3504672‑3504822                                                                           |                                                                             aCCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGc                                                               <  1:432032/90‑1 (MQ=255)           ggCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTc                                                     <  1:423176/90‑1 (MQ=255)                tctGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGtt                                                <  1:459859/90‑1 (MQ=255)                    tttCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCa                                            <  1:162048/90‑1 (MQ=255)                    tttCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCa                                            <  1:327494/90‑1 (MQ=255)                           tACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGa                                     >  1:435840/1‑90 (MQ=255)                                    gacCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCg                            >  1:87943/1‑90 (MQ=255)                                               tGCCTTTGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTc                 >  2:202075/1‑90 (MQ=255)                                                   tttGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGtt             >  1:452712/1‑90 (MQ=255)                                                    ttGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCat                                           >  1:181044/1‑59 (MQ=255)                                                    ttGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCat                                           <  2:181044/59‑1 (MQ=255)                                                    ttGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTc            >  1:111506/1‑90 (MQ=255)                                                    ttGCAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATACCTTTCGTTTAATAACTCGGTTc            >  2:516240/1‑90 (MQ=255)                                                       cAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTg         <  2:200441/90‑1 (MQ=255)                                                       cAGATGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTg         <  2:213106/90‑1 (MQ=255)                                                           tGTCCTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTcc     >  1:403781/1‑90 (MQ=255)                                                              ccTTATCAATAACACCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCca  >  1:112935/1‑90 (MQ=255)                                                                           |                                                                             ACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCA  >  NZ_CP009273/3504672‑3504822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑