| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 333,799 | A→T | G437G (GGA→GGT) | fdrA → | acyl‑CoA synthetase FdrA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 333,799 | 0 | A | T | 90.9% | 21.9 / NA | 11 | G437G (GGA→GGT) | fdrA | acyl‑CoA synthetase FdrA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (5/5); minor base G (1/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACG > NZ_CP009273/333715‑333873 | caccaACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGGTGGGTTTTTTGTTCGGGTTTTGTTCGCa > 2:520030/1‑90 (MQ=255) accaACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGGTCGGGATGGGTTTTTTGGTCGGGTTTGGTTTGCAt > 2:329510/1‑90 (MQ=255) cAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGTTCGCATGAAGATCCGGt < 2:116392/90‑1 (MQ=255) gTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGTTCGCATGAAGATCCGGTCg < 2:12185/90‑1 (MQ=255) ttGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGATGGGTTTTTTGCTCGGGTTTTGTTCGCATGGAGATCCGGTCGGCTcc > 2:15397/1‑90 (MQ=255) gcgcgATCCAGAAGTGGCGGTGATCGGGGTGGATTTTGTGCTCGGGTTTGGGTTGCATGAAGATCCGGGCGGGTCCACCATCGAGACGAt > 1:95609/1‑90 (MQ=255) ggCGGTGATCGTGATGGATTTTTTGCTCGGATTTGGTTCGCATGAAGATCCGGTCGGCTCCCCCATCGAGACGATCAAAAaagcgaaaac > 2:68730/1‑88 (MQ=255) ggCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGTTCGCATGAAGATCCGGTCGGCTCCACCATCgaga > 1:377630/1‑71 (MQ=255) ggCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGTTCGCATGAAGATCCGGTCGGCTCCACCATCgaga < 2:377630/71‑1 (MQ=255) cGTGATGGATTTTGTGCTCGGATTTGGTTCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATcgccgc < 1:178247/90‑1 (MQ=255) tgtgCTCGGATTTGGTTCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGAcg < 1:375118/90‑1 (MQ=255) | CACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACG > NZ_CP009273/333715‑333873 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |