Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,527,973	T→C	M419V (ATG→GTG)	envZ ←	two‑component system sensor histidine kinase EnvZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,527,973	0	T	C	100.0%	45.9 / NA	14	M419V (ATG→GTG)	envZ	two‑component system sensor histidine kinase EnvZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/8);  total (6/8)

TGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAA  >  NZ_CP009273/3527892‑3528032                                                                                  |                                                            tGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGTTAt                                                     <  1:182706/90‑1 (MQ=255) tGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGTTAt                                                     >  2:24314/1‑90 (MQ=255) tGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGTTAt                                                     >  2:364218/1‑90 (MQ=255)       gcgcCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGTTATGGTTAt                                               >  1:119540/1‑90 (MQ=255)        cgcCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGTTATGGTTATc                                              <  1:24314/90‑1 (MQ=255)                ttACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGTTATGGTTATCCACGATAc                                      <  2:361523/90‑1 (MQ=255)                                        gAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGt                                                        >  1:388541/1‑48 (MQ=255)                                        gAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGt                                                        <  2:388541/48‑1 (MQ=255)                                             gAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTg         <  1:9534/90‑1 (MQ=255)                                               aaGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGcc       >  1:167314/1‑90 (MQ=255)                                               aaGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCACCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGcc       <  1:364218/90‑1 (MQ=255)                                                    cgccccgcTCGCTGGTGCCAAGCTCCAGCACCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTaa  <  2:126405/90‑1 (MQ=255)                                                           ctcgctGGTGCCAAGCTCCAGCACCCCGTTATGGTTATc                                              <  1:102941/39‑1 (MQ=38)                                                           ctcgctGGTGCCAAGCTCCAGCACCCCGTTATGGTTATc                                              >  2:102941/1‑39 (MQ=38)                                                                                  |                                                            TGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAA  >  NZ_CP009273/3527892‑3528032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑